兔角膜缘干细胞的形态学研究.docVIP

PAGE 6 PAGE 2 兔角膜缘干细胞的形态学研究＊ 苏敏＊基金项目：贵州省科技厅黔科合J字（2006）082号作者简介： ＊基金项目：贵州省科技厅黔科合J字（2006）082号 作者简介：苏敏（1971年—），通讯作者，女，贵州省遵义市（县）人，汉族，硕士，副教授，主要从事实验组织学方面的研究，发表论文9篇。电话：（0851）6908567，E-mail：summ30@163.com ＊基金项目：贵州省科技厅黔科合J字（2006）082号 （贵阳医学院组织学与胚胎学教研室，（贵州贵阳 550004） 摘要: 目的 探讨兔角膜缘干细胞（Limbal Stem Cells,LSCs）的形态学特征、数量及分布。方法 健康成年新西兰大耳兔30只，体重2.0-2.5kg，雌雄不分，取双侧眼球，常规石蜡切片，HE染色及免疫组化P63染色显示角膜缘一般特征及LSCs的分布，并用BioMias图像分析软件对LSCs进行计数。另外取3只兔眼角膜缘制作超薄切片，透射电镜观察。结果 1.①角膜缘上皮细胞排列紧密，层数较多，基底层细胞呈矮柱状或立方形，核大而圆，位于细胞中央，着色较深。LSCs（P63阳性细胞）呈矮柱状或立方形，多位于角膜缘上皮基底层，在角膜缘浅层及角膜边缘有少量分布，在角膜中央上皮未见LSCs出现。2.②透射电镜观察LSCs核较大，形状不规则，核内常染色质较多，异染色质散在分布，未凝集成块。胞质少，含较丰富的游离核糖体，其它细胞器少，细胞间可见较多的缝隙连接。3.③ 图像分析显示高倍镜视野下角膜缘上皮LSCs数量为35.25±8.93个。结论 1.①LSCs具有原始细胞的超微结构特征，与其他干细胞形态相似。2. ②LSCs主要位于角膜缘上皮基底层，在角膜缘浅层上皮及角膜边缘上皮内也有少量LSCs存在，在中央角膜上皮未见LSCs分布。 关键词 关键词：角膜缘干细胞 角膜缘干细胞；形态学 形态学；P63 P63；超微结构 中图分类号：R329.2 A Morphological Study of the Limbal Stem Cells of Rabbit Su Min Zheng Lin Xu Tingliang Li Hong Hu Rong Huang Yue （Department of histology and embryology of ，Gui Yang medical college, （Gui Yang 550004) ABSTRAC：Objective To investigate the morphological characteristics，quantity and distribution of the limbal stem cells (LSCs) of rabbit. Methods The 30 New Zealand rabbits of that weigh 2？.0-2.5kg were healthy adult male or female. Their eyes were sliced into the paraffin sections.？ We observed the morphological characteristic of the limbus of the cornea with Hematoxylin-Eosin staining. The LSCs were showed by immunohistochemistry technique (P63-staining) and electron microscopy. The P63-positive LSCs were counted with the BioMias software of image analysis. Results 1.The limbal epithelial cells were closely aggregated and the layers of the cells were more than the central zone of the cornea. The cells of the basal layer were low columnar or cubic shape and their nuclei were centrally position and stained darker blue. The P63-positive LSCs appeared low columnar or cubic shape. Many P63-positive LSCs were located in the bottom of the