发光细菌法在黄岛溢油污染海水遗传毒性评价中的应用.docVIP

发光细菌法在黄岛溢油污染海水遗传毒性评价中的应用收稿日期 收稿日期Received: 接收日期Accepted: ?基金项目：科技部科技基础性工作专项，2012FY130300 号；中央级公益性科研院所基本科研业务费专项资金，2014G12号；泰山学者海外创新人才资助 Fund Project : supported by Ministry of Science and Technology of China (2012FY130300), the Basic Scientific Fund for National Public Research Institutes of China (2014G12) and Taishan Scholar Award. 栾晓1 崔志松1??通讯作者：崔志松，博士，副研究员，研究方向：海洋微生物学，Tel: 0532E-mail：czs@fio.org.cnCorresponding Author: Cui Zhisong, Doctor, Associate Researcher, Research Interests: Marine Microb ??通讯作者：崔志松，博士，副研究员，研究方向：海洋微生物学，Tel: 0532E-mail：czs@fio.org.cn Corresponding Author: Cui Zhisong, Doctor, Associate Researcher, Research Interests: Marine Microbiology, Tel: 0532E-mail：czs@fio.org.cn 1国家海洋局第一海洋研究所海洋生态研究中心 青岛 266061 2青岛科技大学化学与分子工程学院 青岛 266042 摘 要 2013年11月22日，黄岛输油管线发生爆炸，胶州湾局部岸滩、水体受到不同程度的溢油污染。本文选取溢油后90d内距离溢油点1-15 km的5个站点，结合GC-MS分析，将一种快速检测遗传毒性的发光细菌Acinetobacter sp. RecA应用于溢油污染海水样品的毒性评价。研究发现，溢油1d后距离泄漏原油入海口1 km以内的S1、S2、S3站点海水样品的石油烃含量为0.55 mg/L～1.04 mg/L，其遗传毒性相当于0.60 mg/L～1.78 mg/L的丝裂霉素C（MMC）；海水中的石油烃含量随时间逐渐降低至＜0.02 mg/L，其遗传毒性也随之逐渐降低。而距离泄漏原油入海口＞5 km的S4、S5站点海水样品的石油烃含量一直较低（＜0.02 mg/L），表现为低毒或无毒。研究表明，使用菌株RecA作为 关键词 黄岛溢油；石油烃；发光细菌；遗传毒性 CLC x824 Genotoxicity evaluation of contaminated seawater in huangdao oil spill based on luminescent bacteria Acinetobacter sp. RecA * LUAN Xiao1, CUI Zhi-Song1**, GAO Wei1, Wen Xiao-long2, Xu Guang-fei2, Li Qian1, SUN Cheng-jun1, Zheng Li1 1Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration, Qingdao, 266061 2 Institute of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, Abstract Objectives: Local beaches and sea water at Jiaozhou Bay was polluted by crude oil to different degrees after the Sinopec pipeline exploded on Nov. 22nd, 2013 at Huangdao, China. In order to investigate the ecological effect of petroleum hydrocarbons in seawater on marine organisms, a rapid genotoxicity detection method based on the