乳腺癌骨转移动物模型的构建张帆姜军杨新华陈庆秋唐鹏作者单位400038第三军医大学西南医院乳腺中心.docVIP

乳腺癌骨转移动物模型的构建 张帆 姜军 杨新华 陈庆秋 唐鹏 作者单位：400038 第三军医大学西南医院乳腺中心 摘要： 目的：建立乳腺癌骨转移动物模型。方法：40只4～6周龄雌性裸鼠随机分为5组，用浓度分别为1×105/ml，1×106/ml，5×106/ml，1×107/ml，5×107/ml的MDA-MB-231细胞悬液0.1ml行左心室注射。观察裸鼠左心室注射致死情况；存活裸鼠49天后行病理检查，确定骨转移发生情况。结果：左心室穿刺注射总致死率为32.50%（13/40）；各组左心室穿刺注射致死率分别为12.5%、12.5%、12.5%、37.5%、87.5%，后三组之间左心室穿刺注射致死率有显著性差异（p＝0.005）。全组骨转移发生率为48.15%（13/27）；各组骨转移发生率分别为：0%、 42.86%、71.43%、80.00%、100%，各组骨转移发生率有显著性差异（p＝0.006）。结论：经裸鼠左心室穿刺注射肿瘤细胞成功建立了乳腺癌骨转移动物模型；肿瘤细胞浓度为5×106/ml时是经裸鼠左心室注射建立乳腺癌骨转移动物模型的最佳浓度。 关键词：乳腺癌；骨转移；动物模型；左心室注射；MDA-MB-231细胞株 An in vivo model of breast carcinoma metastasis in bone ZHANG Fan , JIANG Jun , YANG Xin-hua , CHEN Qin-qiu , TANG Peng (Breast Disease Center , Southwest Hospital , Third Military Medical University , Chongqing 400038 , China) Abstract : Objective To establish animal bone metastasis models of human breast cancer. Methods 40 female nude mice in 4～6 weeks were divided into 5 groups. MDA-MB-231 Cells (1×104，1×105，5×105，1×106，5×107) were suspended in 0.1 ml of PBS and then were injected into the left heart ventricle according to the different groups. Death after injecting into the left heart ventricle was recorded. Bone metastasis of the living nude mice was found by autopsy after 49 days. Results The mortality rate of all nude mice after injecting into the left heart ventricle was 32.50%. The mortality rate of the different groups was 12.5%、12.5%、12.5%、37.5%、87.5%,respectively. There was significant difference in the mortality rate between the last three groups（p＝0.005）. The bone metastasis rate was 48.15%（13/27）. The bone metastasis rate of the different groups was 0%、 42.86%、71.43%、80.00%、100%, respectively. There was significant difference in the bone metastasis rate between the different groups（p＝0.006）. Conclusion We set up an in vivo model of breast cancer metastasis in bone. 5×105 MDA-MB-231 cells suspended in 0.1 ml of PBS was the best concentration to set up an in vivo model of breast cancer metastasis in bone by injecting into