人参总皂苷对K562细胞STAT5表达的影响.docVIP

人参总皂苷对K562细胞STAT5表达的影响国家自然科学基金； 国家自然科学基金；重庆市自然科学基金（2007BB5304） 龙轩* 罗春燕 姜蓉 华自森 王建伟通讯作者，E－mail： HYPERLINK mailto: 通讯作者，E－mail： HYPERLINK mailto:wjwcq@ wjwcq@ （重庆医科大学：*护理学院；基础医学院，重庆市生物化学与分子药理学重点实验室(作者单位标注不清楚)，重庆400016） 摘要 目的: 探讨人参总皂苷（TSPG）对人红白血病细胞株（K562细胞）信号转导与（涵盖太宽）转录因子5（STAT5）表达的影响。方法:免疫细胞化学观察TSPG作用前后K562细胞STAT5表达的变化；以ELISA和Western blotting法检测TSPG处理前后K562细胞的细胞质和细胞核中STAT5含量的变化。结果:免疫细胞化学染色可见对照组K562细胞的细胞质和细胞核着色浅淡；经TSPG 200 mg/L作用12 h后，K562细胞的细胞质和细胞核着色明显加深，表达增强，与对照组相比有显著差异（P0.05）；TSPG （200 mg/L）诱导K562细胞6h、12h、24h后，随着TSPG作用K562细胞时间的延长，细胞质和细胞核中STAT5的表达逐渐增强。结论: TSPG能增强K562细胞STAT5的表达,这可能是TSPG诱导K562细胞向红系细胞分化的作用机制之一。 关键词 人参总皂苷；STAT5；K562细胞 Effect of total saponins of panax ginseng on expression of STAT5 in K562 cells LONG Xuan*, LUO Chunyan, JIANG Rong, HUA Zisen, WANG Jian-Wei （Chongqing Medical University: *nurse medical college；basic medical college, Chongqing key laboratory of biochemistry and molecular pharmacolog y作者单位标注不清楚, Chongqing 400016） Abstract Objective：To investigate the effect of total saponins of panax ginseng on expression of STAT5 in K562 cells. Methods: The expression of STAT5 protein in K562 cells was investigated by immunocytochemistry; STAT5 protein in cytosol fraction and cellular nucleus protein extracts of K562 cells were determinated by Elisa and Western blotting analysis. Results: the cytoplasma and nucleus of K562 cell in control group is weakly stained; after stimulated by 200μg/ml TSPG for 12h, both cytoplasma and nucleus of K562 cell of K562 cell is darkly stained. Compared with control group, the amount of STAT5 in cytoplasma and nucleus of K562 cell induced by 200μg/ml TSPG is increased in time-dependent manner. Conclusion: TSPG can enchance the expression of STAT5 in K562 cell, which may be one of mechanisms by which TSPG induces K562 cells to differentiate into erythroid lineage cells. [Key word]：Total saponins of panax ginseng, STAT5, K562 cell 人参是祖国传统医学的“补气”要药，人参皂苷是人参中主要的有效药物成分。我们既往研究表明，人参总皂苷(total saponins of