京尼平交联对大鼠脱细胞脊髓生物学特性的影响.docVIP

京尼平交联对大鼠脱细胞脊髓生物学特性的影响 蒋 涛，任先军，阴 洪，田永阳，唐北川，王开见 （400037 重庆，第三军医大学新桥医院骨科） [摘要] 目的 探讨新型生物交联剂京尼平对大鼠脱细胞脊髓生物学特性的影响，为脊髓损伤的组织工程研究提供一种理想材料。方法 采用化学萃取法对大鼠脊髓组织进行脱细胞处理，再用生物交联剂京尼平(5 g/L)进行化学交联。分别采用HE染色和扫描电镜观察京尼平交联前后脱细胞脊髓的微观结构，并进行孔径大小、交联率、含水量、在PBS溶液及胰酶溶液中的稳定性能检测，再将大鼠骨髓间充质干细胞分别与京尼平交联前后的脱细胞脊髓浸提液共培养，采用MTT法在体外评估该支架材料交联前后的细胞毒性情况。结果 京尼平交联前后的脱细胞脊髓拥有类似的多孔结构，孔径大小约30 μm，最终交联率可达90％，交联后的脱细胞脊髓含水率从（283.4±11.2）%降至（229.7±12.5）%，但其在PBS及胰蛋白酶中的稳定性得到了明显的增强。交联前后的脱细胞脊髓都未观察到明显的细胞毒性。结论 京尼平交联的脱细胞脊髓部分生物学特性得到改善，为应用于脊髓损伤修复的组织工程材料提供了一种新的 [关键词] 脱细胞脊髓；京尼平；化学交联；细胞毒性 Effect of genipin cross-linked on the biological characterization of the acellular spinal cord in rat JIANG Tao, REN Xianjun, YIN Hong, TIAN Yongyang, TANG Beichuan, WANG Kaijian （ Department of Orthopedics, Xinqiao Hospital, The Third Military Medical University, Chongqing， 400037, China） [Abstract] Objective The in vitro biological characteristics of genipin (GP)-crosslinked acellular spinal cord was investigated in order to provide a kind of ideal biomaterial for tissue engineering application of spinal cord injury. [Method] Rat spinal cord tissues were decellularized by the chemical extraction method, followed by chemical crosslinking with 5g/L genipin solution. 我们也不清楚为什么在这里出现了中文，我们的投稿中没有中文。我们已经删除。The micro-structure of the uncrosslinked and GP-crosslinked acellular spinal cords were observed with HE staining and scanning electron microscopy, and the properties of pore size, crosslinking degree, water uptake ratio, and stability in PBS and tripsin were tested as well. The rat bone marrow mesenchymal stem cell were cultured in lixivium of uncrosslinked and GP-crosslinked acellular spinal cord, and then, the MTT method was used to evaluate in vitro cytotoxicity. [Result] The uncrosslinked and GP-crosslinked acellular spinal cords possessed a similar porous structure with an average pore diameter about 30 μm, the final crosslinking rate was about 90%, the water uptake ratio were decreaseded from 283.4±11.2% to 229.7±12.5% and the stability i