耐药相关基因HA117,mdr1,mrp1,lrp,bcrp在儿童急性白血病中的表达及临床意义.docVIP

耐药相关基因HA117、mdr1、mrp1、lrp、bcrp在儿童急性白血病中的表达及临床意义 郭改丽，徐酉华△ ，伍俞霓，沈亚莉 （重庆医科大学附属儿童医院血液科，重庆400014） 【摘要】目的：探讨HA117、mdr1、mrp1、lrp、bcrp 在急性白血病(AL)患儿骨髓单个核细胞(BMMNC)中的表达及其在AL治疗中的临床意义。方法：采用RT-PCR方法检测81例AL患儿BMMNC中HA117、mdr1、mrp1、lrp、bcrp基因的表达；并以14例免疫性血小板减少性紫癜( ITP)作为对照。结果：① HA117、mdr1、mrp1、lrp、bcrp阳性表达率均高于对照组,难治/复发组五种基因的表达率均高于初诊组及完全缓解组。②HA117、lrp阳性的首次完全缓解率（CR1）率低于阴性的CR1率（P﹤0.05），mdr1、mrp1、bcrp阳性的CR1率低于阴性的CR1（P﹥0.05）。③直线相关分析显示，AL中HA117与mdr1、mrp1、lrp、bcrp表达无明显相关性；mdr1与mrp1、mdr1与bcrp、lrp与bcrp呈正相关（r=0.26、0.308、0.317，P﹤0.05）。难治/复发组中，HA117与mdr1、mrp1、lrp及bcrp呈完全正相关。结论：HA117与mdr1、mrp1、lrp、bcrp可能共同参与了儿童AL耐药的产生，可作为评估化疗效果与预后的一个重要指标。HA117与lrp较mdr1、mrp1及bcrp可能更具有预测MDR的意义。 【关键词】急性白血病；聚合酶链反应；多药耐药；基因表达；化疗 Clinical Significance of HA117、mdr1、mrp1、lrp and bcrp Expression in Children with Acute Leukemia 　 GUO Gaili,XU Youhua△,WU Yuni,SHEN Yali　 Department of Hematology，Children，s Hospital of Chongqing Medical University ,Chongqing 400014，China 【Abstract】Object: To explore the expression of the HA117、 mdr1、mrp1、lrp and bcrp genes in bone marrow mononuclear cells (BMMNC)with acute leukemia and the clinical significance in acute leukemia.Methods: Expressions of HA117、mdr1、mrp1、lrp and bcrp in 81 children with acute leukemia and 14 children with Idiopathic thrombocytopenic purpura (ITP) were tested using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique.Result: ①The expressions of HA117、mdr1、mrp1、lrp and bcrp in AL patients were higher than them in ITP children. And the expressions of these genes in refractory-relapsed patients also higher than them in the initially diagnosed patients or remission patients.②The first complete remission (CR1）rate in HA117 and lrp positive cases was lower than it in negative cases(P﹤0.05), but the CR1 rates in mdr1、mrp1 and bcrp positive cases had no significant difference from it in negative cases.③Correlation analysis showed: There was no correlation between the expression of HA117 and mdr1 or HA117 and mrp1 or HA117 and lrp or HA117