过表达IL-12调节单核系肿瘤细胞向巨噬细胞分化-中国细胞生物学学报.PDF

中国细胞生物学学报 Chinese Journal of Cell Biology 2014, 36(4): 455–460 DOI: 10.11844/cj cb.2014.04.0345 过表达IL-12调节单核系肿瘤细胞向巨噬细胞分化 马婷婷 熊海玉 王 秦 成 凤 李紫薇 吴碧涛 林 艳 涂植光* (重庆医科大学检验医学院, 临床检验诊断学教育部重点实验室, 重庆400016) 摘要 构建过表达白细胞介素-12(interleukin-12, IL-12)的THP-1单核系肿瘤细胞模型, 从形态 学、巨噬细胞表面标志表达水平及吞噬功能3方面探究过表达IL-12能否调节单核系肿瘤细胞向巨噬 细胞分化。结果发现, 与空白和空载组相比, IL-12组的THP-1细胞界限不明显, 散在细胞增多; 呈圆 形或不规则圆形, 偶见毛刺状突起; 核仁模糊或消失, 核染色质条索状浓集; CD68 mRNA和蛋白表达 量均升高(P0.05)。与空白组相比, CD 11b mRNA和蛋白表达量升高不明显(P0.05), 但72 h CD 11b 蛋白表达量明显高于相同培养条件下的空载组(P0.05) 。培养72 h后各组细胞吞噬能力比较, IL-12 组吞噬率(36.7± 1.2)% 高于空白组(15.7±3.1)%和空载组(28.0± 1.5)%, 差异有统计学意义(P0.05) 。 上述结果提示, 过表达IL-12可以促进单核系肿瘤细胞向巨噬细胞分化。 关键词 白细胞介素-12(IL-12); 过表达; 单核系肿瘤细胞; 巨噬细胞; 分化 IL-12 Overexpression Regulate the Differentiation of Monocytic Tumor Cell to Macrophagocyte Ma Tingting, Xiong Haiyu, Wang Qin, Cheng Feng, Li Ziwei, Wu Bitao, Lin Yan, Tu Zhiguang* (College of Laboratory Medicine, Key Laboratory of Laboratory Medical Diagnostics of Education Ministry, Chongqing Medical University, Chongqing 400016, China) Abstract In this study, whether IL-12 overexpression could regulate differentiation of monocytic tumor cell to macrophagocyte was investigated. Firstly, a monocytic tumor THP-1 cell model overexpressing IL-12 was established. Furthermore, the change of cell morphology, matured macrophagocyte marker expressions and phagocytosis were detected. Morphologically, the experimental group cells were round or irregular round shape, and some cell membrane processes were observed; cell nucleolus became fuzzy or completely disappeared; the nuclear chromatins appeared to be dense and cordlike. Compared with the control group and empty vector group, CD68 mRNA and protein expressions were up-regulated (P0.05). However, compared with blan