联系作者罗振华-第三军医大学学报.DOC

PAGE PAGE 3 联系作者：罗振华 通讯地址：贵州省贵阳市北京路9号贵阳医学院微生物学教研室 邮编：550004 单位：贵阳医学院微生物学教研室 手机E－mail:luo8300@ 人工合成家蝇抗真菌肽MAF-1A对白色念珠菌致病性的影响 罗振华1，吴建伟2*，付萍2，国果2，姜珊2 （1.贵阳医学院微生物学教研室，2.贵阳医学院寄生虫学教研室，贵州 贵阳 550004） [摘要] 目的 观察和研究人工合成家蝇抗真菌肽MAF-1A对白色念珠菌致病性的影响。方法 以白色念珠菌ATCC10231为效应菌，检测MIC浓度（0.6mg/ml）MAF-1A在不同时间白色念珠菌的芽生孢子萌发率及芽管形成率；分别采用YEPD及RPMI1640培养基培养获取白色念珠菌酵母相及菌丝相细胞，采用涂片固定瑞氏染色显微镜计数，观察不同浓度MAF-1A预处理后及MAF-1A干预下，酵母相及菌丝相白色念珠菌体外定植人口腔黏膜上皮细胞能力的变化；分别设立氟康唑和PBS为药物阳性对照和阴性对照。结果MAF-1作用白色念珠菌后，作用2h、4h和6h的芽生孢子形成率分别为11.0±2.6%、18.7±2.5%和24.3±3.5%，作用1h、2h和3h的芽管形成率分别为35.0±1.0%、40.0±1.5%、55.7±1.5%，芽生孢子萌发率及芽管形成率小于药物阳性对照组和阴性对照组（P＜0.05）；0.6mg/ml、1.2mg/ml和1.8mg/mlMAF-1A均可减少白色念珠菌酵母相细胞以及菌丝相细胞对人口腔黏膜上皮细胞的粘附数（P＜0.05）； MAF-1A预处理白色念珠菌后，对人口腔黏膜上皮细胞粘附数少于MAF-1A干预组的细胞粘附数（P＜0.05）；无论是预作用组或干预组，MAF-1A减少酵母相细胞粘附作用强于菌丝相细胞（P＜0.05）。结论 MAF-1A可通过抑制白色念珠菌孢子萌发和芽管的形成以及有效降低白色念珠菌对人口腔黏膜上皮细胞粘附能力，从而影响白色念珠菌的致病能力。 [关键词]家蝇；抗真菌肽MAF-1A；白色念珠菌，致病性 Effect of the Synthetic Musca domestica Antifungal Peptide-1A (MAF-1A) on the Pathogenicity of Candida albicans Luo Zhen-hua, Wu Jian-wei*, Fu ping, Guo Guo, Jiang Shan （Guiyang Medical college,Guiyang,Guizhou 550004,China） [Abstract] Objection To probe the effect of the synthetic Musca domestica antifungal peptide-1A(MAF-1A) on the pathogenicity of Candida albicans. Methods Blastospore germination and germ tube formation of Candida albicans ATCC10231 were detected, yeast form and hyphal form of Candida albicans were cultivated in YEPD medium and RPMI1640 medium respectively and the yeast form and hyphal form numbers of adherence to BEC in vitro were counted using Wrights staining after exposing to MIC concentration（0.6mg/ml）of MAF-1A, with fluconazole and PBS as positive and negative controls. Results The spore formation rates of Candida albicans treated with MAF-1A were 11.0±2.6 percent, 18.7±2.5 percent and 24.3±3.5% percent cultured for 2h, 4h and 6h, the germ tube formation were 35.0±1.0 percent, 40.0±1.5 percent and 55.7±1.5% percent cultured for 1h, 2h and 3h.Compar