基质金属蛋白酶2在胃癌组织中的表达与临床意义.docVIP

基质金属蛋白酶2在胃癌组织中的表达与临床意义 李志贵1，关泉林2（641000，内江，四川省内江市第二人民医院肿瘤外科1；730000，兰州，兰州大学第一附属医院肿瘤外科2） The expression of MMP-2 in Li Zhigui,Guan Quanlin 1Department of Oncological Surgery ,Neijiang Second Pepple’s Hospital,neijiang,641000,China . 2Department of Oncological Surgery ,First Affiliated Hospital, Lanzhou University,Lanzhou 730000,China Abstract： Objective To investigate the expression of matrix metalloproteinase-2(MMP-2) in human gastric neoplasms and its significance. Methods A total of 41 specimens resected was investigated by immunohistochemical staining and real-time fluorescence quantitative PCR. The expression of MMP-2 were detected in various regions of gastric neoplasms，adjacent normal tissues，incisal margin normal tissues.The expression of MMP-2 and clinicopathological factors were analyzed. Results 　The positive ratios of MMP-2 in gastric neoplasms ，adjacent normal tissues，incisal margin normal tissues were 60.98%(25/41) and 9.76%(4/41)，0%（0/41），respectively. The expression of MMP-2 mRNA compared to incisal margin normal tissues in various regions of gastric carcinomas,adjacent normal tissues were 1.43 ± 0.47,1.17 ± 0.72. The expression of MMP-2 correlated with clinical stage，lymph node metastasis. Conclusion The expression of MMP-2 in gastric neoplasms is strong. Strong expression of MMP-2 are associated with t Key words： gastric neoplasms；matrix metalloproteinase-2 ；immunohistochemical staining 摘要： 目的 探讨胃癌患者肿瘤组织、癌旁正常组织及手术切缘正常组织中基质金属蛋白酶2（MMP-2）的表达与临床病理特征的关系。方法 采用免疫组织化学技术检测MMP-2蛋白，实时荧光定量PCR检测MMP-2 mRNA在41例胃癌组织及相应癌旁正常组织、手术切缘正常组织中的表达，及与胃癌临床病理特征之间的关系。 结果 MMP-2蛋白在胃癌组织、癌旁正常组织、手术切缘正常组织中的阳性表达率分别为60.98%(25/41)、9.76%(4/41)、0%（0/41）；MMP-2 mRNA肿瘤组织中表达量是手术切缘组织的1.43±0.47，癌旁正常组织是1.17±0.72，癌旁正常组织与手术切缘正常组织无明显差异。MMP-2蛋白及 mRNA 表达水平与性别、年龄、肿瘤部位、浸润深度、细胞分化级别无关，与有无淋巴结转移、临床分期密切相关。 结论 MMP-2在胃癌组织中高表达，并且与肿瘤的恶性程度密切相关，有可能成为判断胃癌预后的标志物及基因治疗的潜在靶点。 关键词： 胃肿瘤；基质金属蛋白酶2；荧光实时定量酶链式反应；免疫组织化学 中图法分类号：R735.2 文献标示码：A 胃癌是消化道常见肿瘤之一，在我国的发病率和死亡率居各种恶性肿瘤的首位，年平均死亡率为25.53/10万[1]。胃癌早期以手术切除为主，根