间充质干细胞尿路上皮细胞—脱细胞基质修复尿道缺损.docVIP

间充质干细胞/尿路上皮细胞—脱细胞基质修复尿道缺损 冯春祥 范应中 孙生安 李泸平 张胜利 李浩 王文举 摘要：目的：对间充质干细胞—基质复合材料、尿路上皮细胞—基质复合材料与单纯脱细胞基质材料修复尿道缺损效果的差异进行了初步探讨。方法：取离体培养第4代尿路上皮细胞和骨髓间充质干细胞（BMSC）做为种子细胞，行5-溴脱氧尿嘧啶核苷(BdrU)标记后接种至脱细胞基质。分别将复合有上皮细胞、BMSC和空白的基质材料移植到尿道缺损动物模型体内，标记为A、B、C组。术后第1、2、4、12周采集动物修复尿道，行组织学观察。术后12周对实验动物行逆行膀胱尿道造影检查。结果：第1周，A、B两组可见稀疏的单层上皮样细胞覆盖，但A组细胞形状较规则。第2周，A、B两组大仍是单层尿道上皮覆盖。A组上皮细胞的致密度较高。第4周，A、B两组上皮细胞分布与正常组织较接近，但A组细胞分层较好。第12周，各组新生尿道与正常组织已明显区分。C组术后并发症发生率为80%，高于另外两组。结论：1.BMSC或尿路上皮细胞复合脱细胞外基质移植到机体内可有效修复缺损尿道，并且效果优于单纯脱细胞外基质材料。2.接种至第4周，发现尿路上皮细胞复合脱细胞基质材料修复尿道效果较BMSC好。 关键词：间充质干细胞 尿路上皮细胞 脱细胞基质 尿道缺损修复 Urethral Reconstruction by Mesenchymal Stem cell/ Urothelium - Acellular Extracellular Matrix FENG Chun-xiang, FAN Ying-zhong, SUN Sheng-an, LI Lu-ping, Zhang Sheng-li, Li Hao, Wang Wen-jun Objective：Comparing the repair results difference, among stem cell - acellular extracellular matrix、urothelium - acellular extracellular matrix and acellular extracellular matrix. Provide theoretical basis for the choosing of urethral tissue engineering seed cells. Methods: urethroplasty were performed with acellular extracellular matrix(group C),acellular extracellular matrix seeded with urothelium (group B), acellular extracellular matrix was seeded with BMSC (groupC).The newborn urethral tissue was cut regularly (in 1、2 、4 、12 w after surgery) for histological、immunochistochmeistry examination to compare their differences. Cell had been preformed BrdU label before seeding into group A and group B. We evaluated the regeneration by urethrography in 12w. Results: In 1w , groupA、groupB could be seen thin layer urothelium coverage in repair region. However most region of the groupA could be seen covered by urothelium junction.; in 2w, group A and group B could be seen the similar uorhtelium distribution, but groupB was better in the density of urothelium. In 4w, the urothelium of group A and B was close to normal, and the distribution of groupA was better than group B. In 12w，the urothelium distribution in repair region of each