脂联素预处理对大鼠肝脏热缺血再灌注胆管损伤的保护作用研究.docVIP

脂联素预处理对大鼠肝脏热缺血再灌注胆管损伤的保护作用研究请作者通读全文，编辑处理较多多字、少字、别字情况，注意语句前后逻辑关系 请作者通读全文，编辑处理较多多字、少字、别字情况，注意语句前后逻辑关系 夏 洋，龚建平( 400010 重庆， 重庆医科大学附属第二医院肝胆外科) [摘要] 目的 观察脂联素对肝脏热缺血再灌注胆管损伤的保护作用及其对细胞凋亡的影响，探讨其中可能的保护机制。方法 将36只大鼠按随机数字表法分为3组：空白组、缺血再灌注组、脂联素处理组，每组12只。术后3、6、12、24 h采取血清及肝组织，采用ELISA法分别测定血液中IL-6浓度及TNF-α浓度；PCR法测胆管组织IL-10及TNF-α mRNA；Western blot法检测各组Capase-3蛋白含量；TUNEL法测定各组胆管细胞凋亡情况；测定血清谷丙转氨酶(ALT)、碱性磷酸酶(ALP)浓度、总胆红素（TBIL），并进行肝脏组织病理学检查。结果择重补充正文结果中的数据，注意与正文数据一致，英文摘要相应修改 缺血再灌注组和脂联素处理组血清ALT、ALP、TBIL、IL-6和TNF-α水平明显高于空白组，并且两组间差异有统计学意义（P0.05）。与缺血再灌注组相比，脂联素处理组肝脏组织炎症因子IL-6及TNF-α减少，IL-10增高，胆管细胞凋亡明显减少（P0.05）。缺血再灌注组与脂联素处理组的Caspase-3蛋白表达量和胆管细胞凋亡指数的水平差异有统计学意义（P0.05）。结论 大鼠肝脏缺血再灌注后胆管损伤加重，脂联素降低炎症反应，减少胆管细胞凋亡，对缺血再灌注后胆管损伤有一定保护作用。 择重补充正文结果中的数据，注意与正文数据一致，英文摘要相应修改 [关键词] 脂联素；缺血再灌注；炎症；胆管细胞；凋亡 [中图法分类号] R392.11; R392.4; R657.3 ［文献标志码］ A The Protective Eeffect of Aadiponectin Ppreconditioning on Bbile Dduct Iinjury in rats of after aAcute warm Hhepatic Iischemia-Rreperfusion in Rats Xia Yang, Gong Jianping ( Department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China) [Abstract] Objective To observe determine the therapeutic effects of adiponectin on liver warm ischemia reperfusion injury (IRI) and on the apoptosis of bile duct cells in rats, apoptosis in liver ischemia reperfusion injury (IRI) and clarify its possible mechanism. Methods SD rats were randomly divided into three 3 groups (n=12 in each group):, Sham sham group, IRI group and adiponectin preTtreatment group (60 μg/kg before IRI) (12 in each group)．. Blood and hepatic tissue samples have beenwere harvested at 3h, 6h, 12h, and 24 h after reperfusion. Serum levels of Ttumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) concentrations were measured by ELISA in blood. Their umor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) mRNA levels in bile duct tissues were measured by PCR in bile duct tissues. TUNEL technique was carried out to determine apoptotic cells in situ in