纤维粘连蛋白EDA片段调控结肠癌SW480细胞增殖,凋亡的研究.docVIP

纤维粘连蛋白EDA片段调控结肠癌SW480细胞增殖、凋亡的研究 耿培亮，欧娟娟，萨日娜，何金霞，谢赣丰，刘平，梁后杰基金项目：国家自然科学基金 基金项目：国家自然科学基金 Supported by the National Natural Science Foundation of China 作者简介：耿培亮，硕士生，医师。主要从事肿瘤分子靶向治疗和化疗耐药方面的研究。电话通讯作者：梁后杰，Email:lianghoujie@ 摘 要: 目的 研究纤维粘连蛋白EDA片段对结肠癌SW480细胞增殖凋亡的影响，为寻找恶性肿瘤治疗的新靶点提供理论基础。方法 利用慢病毒载体沉默结肠癌SW480细胞的纤维粘连蛋白EDA片段, 建立纤维粘连蛋白EDA片段低表达的SW480细胞。利用MTT检测沉默前后细胞活性的变化，并使用流式细胞术检测沉默前后细胞周期和细胞凋亡的变化。同时使用Western blot分别检测转染前后凋亡相关蛋白Bax和Bcl-2的表达变化。结果 沉默纤维粘连蛋白EDA片段的细胞对比未沉默及转染空质粒的细胞，沉默后SW480细胞的活性明显降低，同时凋亡明显增加。Western blot的结果显示沉默纤维粘连蛋白EDA片段后促凋亡因子Bax表达上调，同时，凋亡抑制因子Bcl-2表达降低。结论 纤维粘连蛋白EDA片段结肠癌SW480细胞具有促增殖、抗凋亡作用，有望成为恶性肿瘤靶向治疗的新靶点。 关键词：纤维粘连蛋白；RNAi；结肠癌；增殖；凋亡 The HYPERLINK /study/ \t _blank Study of fibronectin EDA regulate proliferation and apoptosis in colorectal cancer SW480 cell Geng Peiliang，Ou Juanjuan，Sa Rina，He Jinxia，Xie Ganfeng, Liu Ping，Liang Houjie （Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China） Abstract：Objective: To investigate the effects of fibronectin EDA on proliferation and apoptosis of human colorectal cell line SW480 and to provide experimental date for clinic utilization. Methods: Lentiviral Vector silences fibronectin EDA. MTT was uesd to analyse cell viability. Cell apoptosis and cell cycle was analyzed by flow cytometry. Results: As compared with the parental cell and negative control cell, the inhibition of fibronectin EDA can inhibit the proliferation and induce the apoptosis of human colorectal cacer SW480 cells. Western blot method showed that the expression of Bax was increased，and the expression of Bcl-2 was decreaseed. Conclusion: Fibronectin EDA can induce the proliferation and inhibit the apoptosis of human colorectal cacer SW480 cells. The inhibitor of Fibronectin EDA may be a HYPERLINK /new/ \t _blank new HYPERLINK /target/ \t _blank target in the HYPERLINK /treatment/ \t _blank treatment of the malignant tumors. Key words: Fibronectin; RNAi; Colorecta