恶性症原虫红内期两个重要候选抗原的相互影晌.PDFVIP

第二军医大学学报 2004 Jan;250) • 18 • Acad J Sec Mil Med Univ • -ì公著· 恶性症原虫红内期两个重要候选抗原的相互影晌 钱锋，张青锋，薛向阳，潘卫庆祷 (第二军医大学基础医学部病原生物学教研室，上海 200433) [摘要] 町的:分析恶性症原虫红内期 2 个疫苗候选抗原 PfAMA-IC][ )和 PfMSPl-19 的相互关系。方锋:用 ELISA 和竞争 ELISA 检测蛋白和抗体的反应，一些抗体用特定的蛋白进行预吸附。结采:PfMSPl-19 蛋白能去除恶性芜疾患者血浆对 PfAMA-IC][ )的反应，这种能力是剂量依赖的，完全去除需要高的蛋白浓度 ;PfMSPl-19 蛋白也能去除兔抗 PfCP-2.9 血清、 兔抗 PfAMA-lC ][ )血清、亲和纯化的兔抗 PfAMA-l( ][ ) IgG 对 PfAMA-IC][ )的反应。结伦:酵母表达的 PfMSPl-19 重组 蛋白能吸附 PfAMA-IC][ )特异抗体。 〔关键词] 恶性症原虫;红内期;酶联免疫吸附测定 [中图分类号] R 382.31 〔文献标识码] A [文章编号] 0258-879X (2004) 01-0018-04 Interaction between 2 major vaccine candidates at blood-stage of Plasmodium falciparum QIAN Feng.ZHANG Qing-Feng.XUE Xiang-Yang.PAN Wei-Qing 幡 (Department of Etiological Biology. College of Basic Medical Sciences .Second Military Medical University.Shanghai 200433.China) [ABSTRACT] Objective: To analyze the relationship between PfAMA-1 ( ][ ) and PfMSPl-19. 2 erythrocyte stage major vaccine candidates of Plasmodium /alci户arum. Methods: ELISA and competitive ELISA were used to examine the interaction between proteins and antibodies. Some antibodie, s were pre-adsorbed. Results: PfMSPl-19 protein had ability to alleviate the reaction of one plasma sample from a falciparum patient with PfAMA-1 ( ][ ) .and it was dose-dependent and high concentra tion was needed for elimilation of the reaction. PfMSPl-19 protein also had the ability to alleviate the reaction of rabbit anti PfCP- 2. 9 serum. rabbit anti-PfAMA- l( ][ ) serum and affinity purified rabbit anti-PfAMA•l(][) IgG with PfAMA- l(][). Conclusion: Recombinant PfMSPl-19 produced in Pichia 户astoris has the ability to adsorb anti-pfAMA- lC