小桐子枝叶抑菌活性部位分离及其GC-MS分析-广东农业科学.PDFVIP

广东农业科学 2013 年第18 期 91 桐子枝叶抑菌活性部位分离及其GC-MS分析 李育川 李维莉 莫丽玲 靳 松 渊昆明学院农学院 云南 昆明 650214冤 摘要 以具有抑菌活性的小桐子枝叶乙醇粗提物的石油醚萃取相为试验材料 采用硅胶柱反复柱层析 结合PTLC分离制备 同时对各阶段分离产物进行活性追踪 筛选出具有抗菌作用的活性部位AJ3 采用GC-MS分析 初步鉴定出活性部位的主体成分遥 结果表明 AJ3 组分在浓度为0.8 mg/mL 时 对白菜黑斑病菌 也A lternaria brassicae (Berk.) Sacc.页 和小麦赤霉病菌渊Fusarium graminearum冤菌丝72 h生长抑制率均大于85% 与广谱性杀菌剂多菌灵渊carbendazim冤无显著差异 表现出极高的抑菌活性遥对AJ3组 分进行GC-MS分析 鉴定出含量最高的10个化合物 它们占总量的83.52%遥 含量最高的前5位成分依次是3-已(烷)基过氧氢酸尧2- 环,3 [1,3-苯二氧基-5-乙基] -2-丙烯酸尧2,3-环氧-2- 甲基已烷尧2,4-二甲基-3-已醇和苯乙烯 5种主体成分的相对总含量为 64.67% 主体成分属酚酸类尧单萜类化合物遥 关键词 小桐子枝叶曰抑菌活性物质曰分离鉴定曰GC-MS 中图分类号 Q939.96 文献标识码 A 文章编号 1004-874X (2013) 18-0091-03 Isolation and GC-MS analysis of antibacterial activity franctions from Japropha curcas L. branch-leaves LI Yu-chuan, Li Wei-li, Mo Li-ling, J in Song (College of A gric ulture , Kunming Unive rs ity, Kunming 650214, China) Abstract: To creened antibacterial activity components from Jatropha Curcas L. branch - leaves. Using alcohol crude extract of petroleum ether partitioned extracts in J. curcas L. branch- leaves as an experimental material by using silica gel column chromatography, combining with PTLC preparation. At same time, each stage of separated products was carried out by tracking activity, and analyzed by GC- MS, combined with sceening the main compositions of exhibited active components. The results show that with the concentration of AJ3 colony of 0.8 mg/mL, the growth inhibition rates of