COX-2抑制剂塞来昔布对胶质细胞OLN93与胶质瘤细胞U373放射增敏效应的初步分析.pdfVIP

英文摘要 COX-2 抑制剂塞来昔布对胶质细胞OLN93 及胶质瘤细胞U373 放射增敏效应的初步研究 cell was more obvious. 3. The outcome of cell scratch experiment: The migration ability of both OLN93 cell and U373 cell became weakened with the effect of Celecoxib of 40µM, and the inhibition effect to U373 cell was more obvious. 4. The outcome of cell colony formation: Celecoxib of 40μM couldn’t increase the radiation sensitivity of OLN93 cell whose SER=1.04; but could increase the radiation sensitivity of U373 cell whose SER=1.27. 5. The outcome of Flow cytometric analysis : Celecoxib of 40μM didn’t affect the cell cycle distribution of OLN93 cell, but increased U373 cell’s proportion in G /M phase ; 2 Ray could increase OLN93 cell and U373 cell’s proportion in G /M phase; Celecoxib 2 couldn’t induce Ray to increase OLN93 cell’s proportion in G /M phase, but could induce 2 Ray to increase U373 cell’s proportion in G /M phase. 2 Conclusions: 1. Selective COX-2 inhibitor Celecoxib could inhibit the growth of normal glial cell OLN93 and glioma cell U373 as well as their ability of migration to a certain extent. 2. Celecoxib couldn’t enhance radiosensitivity of normal glial cell OLN93, but has a radiosensitization effect on glioma cell U373. 3. Celecoxib regulates glioma cell cycle distribution and increases cell proportion in G /M phase, which maybe one of the mechanisms of enhancing radiosensitivity. 2 4. Celecoxib, a COX-2 selected inhibitor, could be widely clinical used as an effected radiosensitizer. Keywords: COX-2 inhibitor, Celecoxib, r