华蟾素联合吉西他滨对人nsclc细胞的作用研究-外科学专业论文.docxVIP

Study Study on the effect of human NSCLC cell with the conjoin of cinobufotalin and gemcitabine Abstract：Object i vewe will use cinobufotalin and gemcitabine to investigate the human NSCLC HCC827 cell by the subcultureing、drug sensitive test of the thiazolylblu and flow cytometry,to assay tumor cell apoptosis and cell line．By this way，we hope to approach the designation in curing human NSCLC，the influence of its suppression ratio and cell line． Furthermore，we wish to detect the theoretical basis for the cinobufotalin to become the assistant medicament in treating human NSCLC．Nethods： Experience group We utilizate the cinobufotalin and gemcitabine to investigate the HCC827 cell by the in vitro culture、drug sensitive test of the thiazolylblu and flow cytometry,to assay the tumor cell rejection and cell line． Control group 1：We use the gemcitabine to investigate the HCC827 cell by the in vitro culture、drug sensitive test of the thiazolylblu and flow cytometry,to assay the tumor cell inhibition and cell line．Control group 2：We use the cinobufotalin to test the HCC82 7 cell by the in vitro culture、drug sensitive test of the thiazolylblu and flow cytometry，to assay the tumor cell inhibition and cell line．Results：First：we can get the outcome of the gemcitabine in curing human NSCLC，which is the high drug level，the high cancericidal and up suppression ratio，the low survival quotiety．For example，within the scope and range of 0．0025 to O．04 uM，about 1 0％of the cell survival rate decreased,while within the range of 0．08 to O．32 uM about 20％cell survival ．3． 万方数据 西南医科大学硕士学位论文rate 西南医科大学硕士学位论文 rate decreased．Then，we can get the influence of the cell line by the gemcitabine in curing human NSCLC，which can promote cell DNA synthesis and cell mitosis(cell proliferation)，but it induced the apoptosis of cells， resulting the decrease in the number of cells．Second：we can get the result of the cinobufotalin in curing human NSCLC in a concentration-dependent way． That is the high drug