基因工程改良番茄的抗病性的研究-微生物学专业论文.docxVIP

基因工程改良番茄的抗病性的研究攘要 基因工程改良番茄的抗病性的研究 攘要 转揪辫莽NPRI蒸觳爵壤植糊广泛瑷强疾演攘牲，尤其是系统获 得性抗性(SAR)，刺激来感染植!I翱弓l发防御殿应。为通过遗传工程撼 嘉番茄妁疾抗瘸性和酣病性，本研究逯过壤瘘农杼夔介毋，游NPRl 基因导入瓣茄。通过卡那抗性筛选，共获褥12个外植体。透过陶R 验证和Southern杂交分析，证明NPRl基嗣也成功导入3株转基因 攘株孛。瓣这3檬转蕊因檀拣擞了CMV獍悭试验，实验缝莱耋示， 植株没有瘸匠习症出现。这个结撤证实了，NPRl基因舆宥正I訇调撼 霭鲢搪系统获褥撬瞧裘达酶臻畿。 关键词：番茄撤熄农杆菌系统获得性抗性NPRI基因，OMV Genetic Genetic Modification to Enhance Tomato＆sistance to Disease Abstract The Arabidopsis NPRl gene controls the onset of systemic acquired resistance(SAR)，a plant immunity that is often activated after a local infection．Once established,SAR can provide the uninfected parts of plants a resistance to a broad spectrum of pathogens。To genetically engineer a broader spectrum of enhanced disease-resistance／tolerance in tomato plant,NPRl was introduced into a tomato usi憋the Agrobacterium tumefaciens mediator method．Twelve explants were selected by Km，PCR and Southern blotting results confirmed that three new plants were transformed．The transformed plants were assayed for resistance to CMV,confirmed that the resistance against pathogen infection in transformed plant was increased significanfl)；this work confirmed that the NpRi could positively regulate the expression of tomato systemic acquired resistance and this gene can be used for engineering for resistance to disease． Key word：Tomato，Agrobacterium tumefaciens,systemic acquired resistance．CMV,NPRl gene． ——一一 ——一一 ￡堑查矍鬓主兰垄望奎 Genetic Modification to Enhance Tomato Resistance to Disease l。Introduction Plant biotechnology ushers in a new era for plant scientists working to maintain he8lthy plants，optimize crop yields，and minimize pesticide application。One of the ultimate aims ofagricultural biotechnology is to feed an expanding world population． A recent survey by The Economist shows that the world pepuladon has increased by 90％in the past 40 years while food production has increased by only 25％per head． With鲢additional 1．5 billion mouths to feed by 2020。farmers worldwide will have to produce 39％more grain(The E