医学毕业姜黄素对雄激素非依赖性前列腺癌pc3细胞凋亡影响.docxVIP

XX大学 毕业论文 姜黄素对雄激素非依赖性前列腺癌PC 3细胞凋亡影响 姓 名： 2014年6月25日 作者：齐瑞芳于小玲尚芳芳赵辉 【摘要】 目的观察姜黄素对雄激素非依赖性前列腺癌细胞株PC 3细胞增 殖和凋亡的影响。方法 分别用0、10、20、30和40 pmol/L姜黃素作用PC 3 细胞48 h后，Cell Counting Kit 8(CCK8)法检测细胞存活率；选用0、5、10、 20 gmol/L姜黄素作用PC 3细胞48 h后，Hoechst染色观察PC 3细胞形态 的变化，Annexin V/PI检测细胞凋亡率。结果 姜黄素能显著抑制PC 3细胞的 增殖(F=36.82, q=4.88?15.36,P0.01);除0与5 gmol/L姜黄素组细胞凋亡率比较 差异无显著性外，不同浓度姜黃素组之间细胞凋亡率比较差异有显著性 (F=7.8q=4.15?6.54,P0.05)。20 ymol/L 姜黄素作用 PC 3 细胞 48 h 后，倒置显 微镜下可观察到部分凋亡细胞出现染色质凝集、核I占I缩、核碎裂等形态学改变。 结论姜黄素能显著抑制体外PC 3细胞的增殖，诱导细胞凋亡，为其应用于临 床雄激素非依赖性前列腺癌的治疗提供了实验依据。 【关键词】 姜黄素；前列腺肿瘤；PC 3细胞；细胞凋亡 [ABSTRACT] Objective To observe the effect of curcumin on proliferation and apoptosis of PC 3 cells in non androgen dependent prostate cance匚 Methods Different concentrations of curcumin 0, 10, 20, 30 and 40 |imol/L were used to act on PC 3 cells for 48 h, the survival rate of the cells was then determined using Cell Counting Kit 8 (CCK8); 0,5,10, and 20 |imol/L curcumin were used to act on PC 3 cells for 48 h and then the cell morphology was observed by Hoechst staining. Annexin V/PI was applied for apoptosis detection. Results Curcumin could significantly inhibit the PC 3 cell proliferation (F=36.82,q=4.88-15.36?P0.01). The differences of the cell apoptosis rates in different concentration groups were significant, except for the 0 and 5 gmol/L groups (F=7.88,q=4.15 —6.54,P0.01). After acting with 20 (imol/L curcumin for 48 h, the morphological changes such as chromatic agglutination, karyopyknosis, and nuclear fragmentation could be seen in part of apoptotic cells with inverted microscope. Conclusion Curcumin could, in vitro, dramatically inhibit PC 3 cell proliferation and induce apoptosis, which provides an experimental evidence for therapy of non hormone dependent prostatic cance匚 ［KEY WORDS］ Curcumin; Prostatic neoplasms; PC 3 cells; Apoptosis 姜黄素是从多年草本植物姜黄中提取的一种酚类色素，它具有消炎、抗氧 化、抗动脉粥样硬化和降血脂等作用［1］。近年来，随着医学与分子生物学的发 展，人们对姜黄素的抗诱变及抗癌作用有了进一步认识［2］。姜黄素的抗肿瘤机 制是多方面的，其中诱导肿瘤细胞凋亡的机制受到人们广泛的关注。本实验选用 雄激素非依赖性前列腺癌细胞株PC 3细胞作为研究对