缺血后处理对脑缺血再灌注大鼠Akt表达的影响与水蛭注射液的干预.pdfVIP

后处理组(P 0.05) ，也低于水蛭后处理组（P 0.05 ）。(2)各组 Akt 蛋白磷酸化水平的比较：假手术组有少量蛋白表达；与假手术组比较, 对照组蛋白表达明显升高（P 0.05 ）；与对照组比较，缺血后处理组、 水蛭后处理组、联合后处理组Akt 蛋白磷酸化水平均升高(P 0.01) ， 其中联合后处理组蛋白磷酸化水平更为显著(P 0.01) 。(3)Akt mRNA 表达的比较：假手术组有少量Akt mRNA 转录；与假手术组比较,对 照组Akt mRNA 转录水平有所升高（P 0.05 ）；与对照组比较，缺血 后处理组、水蛭后处理组、联合后处理组Akt mRNA 转录水平均升高 (P 0.01)，联合后处理组 Akt mRNA 转录水平既显著高于缺血后处 理组(P 0.01) ，也高于水蛭后处理组（P 0.01 ）。 结论： 脑缺血后处理可能以促进大鼠信号通路中关键信使 Akt 转录及其蛋白表达，发挥神经保护作用，水蛭注射液可增强该效应。 关键词： 水蛭,水蛭后处理，缺血后处理，蛋白激酶B Effect of ischemic postconditioning on expressi- on of AKT following cerebral ischemic/re- perfusion in rats and inervention of hirudo mendicinlies ABSTRACT Objective ： In this article, base on AKT signal transduction pathway method, we discuss how leech injection IPOC combine IPOC effect on mechanism of rat’ cerebral protection. Also, we discuss leech injection clinical application on experimental foundation level. I believe my discussion will improve treatment of ischmia/reperfusion on both of that trends : First, application of traditional Chinese medicine; second, intergration of Chinese traditional medicine and Western medicine. Methods: 60 male rats were assigned into five groups randomly . Those group are named as: sham-operation group (SO); I/R group (C); IPOC group (P); leech Injection group(L-H); and leech injection combine IPOC group (P-H).I experiment SO group as following:First step; Performing an opertation without ischemic surgery. Second step: Injecting a 4ml normal saline intraperitoneal injection and performing step one simultaneously.I experiment C group as following: First step: Performing an ischemic surgery for 90 minutes.Second step: Performing an