华佗外敷麻药神方加减方治疗慢性湿疹的实验研究-方剂学专业论文.docxVIP

— — PAGE 1— 摘 要 华佗外敷麻药神方加减方治疗慢性湿疹的实验研究 研究生：于霞 导师：周光 教授 摘 要 目的：本研究旨在观察华佗外敷麻药神方加减方治疗慢性湿疹的作用，并通过 小鼠动物模型探索药效及其作用机理。方法：采用二硝基氯苯（DNCB）激发的方法 诱发小鼠耳部慢性湿疹模型，比较华佗外敷麻药神方加减方与其他对照组之间的差 异；观察华佗外敷麻药神方加减方对小鼠耳肿胀度、耳组织病理学变化和炎症细胞 浸润情况及脾脏指数、胸腺指数等的影响。结果：华佗外敷麻药神方加减方可显著 抑制 DNCB 所致小鼠皮肤迟发性变态反应引起的耳肿胀，能减少耳组织浸润炎性细 胞数，与阳性药对照组丁酸氢化可的松乳膏相比，作用相当(P0.05)。华佗外敷麻药 神方加减方组与丁酸氢化可的松组均能使小鼠胸腺指数显著降低，与模型对照组相 比，差异有统计学意义(P0.05)，华佗外敷麻药神方加减方组与模型对照组相比不能 降低小鼠脾指数(P0.05)。结论：华佗外敷麻药神方加减方治疗慢性湿疹疗效肯定， 具有良好的抗炎和抗变态反应作用，其作用与丁酸氢化可的松乳膏相当，能显著降 低小鼠慢性湿疹的胸腺指数，其机制可能与组方中部分中药的药理作用即调节免疫 作用有关。 关键词： 华佗外敷麻药神方加减方；慢性湿疹；实验研究；动物；外敷 新疆医科大学医学硕士学位论文 Experimental study of Huatuo external applyment anesthetics Shen prescription Jiajian prescription in treating chronic eczema Postgraudate:Yuxia Supervisor: Professor. Zhou Guang Abstract Objective: This research is to observe the effect of Huatuo external applyment anesthetics Shen prescription Jiajian prescription in treating the chronic eczema，and study tne drug efficacy and therapeutic mechanism in mice eczematous.Methods: Repeated challenges of the ears of previously sensitized mice with 2,4—dinitrochlorobenzol(DNCB) induced typical chronic eczema model, to compare the difference between it and other Model compare group. The influences of it on ear swelling and ear tissue pathology induced by dinitro chlorobenzone (DNCB) were observed in mice,the difference of index of spleen and index of thymus.Results：Huatuo external applyment anesthetics Shen prescription Jiajian prescription is effective reaction on anti-delayed allergy reaction of skin in mice .and it can relieve the ear swelling and significantly too inflammatory cellular infiltration in epidermis and dermis of mice,compared with it in (the positive control group)hydrocortisone group(P0.05),Calculation results of index of thymus indicated both in Huatuo external applyment anesthetics Shen prescription Jiajian prescription group and hydrocortisone group significantly reduced,compared with it in Model compare group(P0.05),index of spleen ind