兽疫链球菌GntR家族中gntR4基因功能的研究-轻工技术与工程（发酵）专业论文.docxVIP

ABSTRACTStreptococcus ABSTRACT Streptococcus zooepidemicus is the main strain for industrial fermentation production of hyaluronic acid(HA)．The GntR family is an important family in the super families of transcription regulator，and it is ubiquitous in the prokaryote．It participates in regulating the biological processes and the pathogenic processes，and it is essential to growth and development of the cell．The GntR family of transcription regulators in Bacillus subtilis and streptomyces coelicolor have been well known，but still remain dark in S zooepidemicus． Streptococcus equi subsp．zooepidemicus ATCC39920 was used as the object of study． The main results were as follows： (1)Study the development relationship of the S zooepidemicus GntR family protein system，and determine the classification of the GntR protein subfamily Searched the protein sequences of GntRl．9，and used the clu-x and MEGA to construct a phylogenetic tree，and the result proved that GntR 1 and GntR7 belonged to FadR subfamily，GntR2，GntR3 and GntR8 belonged to HutC subfamily，GntR6 belonged to MocR subfamily，GntR4 belonged to YtrA subfamily，and GntR5 and GntR9 did not belong to these four types． (2)Study the function of the∥坎gene，and determine the role that the肜堰plays in the S zooepidemicus We used the markerless gene deletion system to successfully delete the nine朋缓 genes of the s．zooepidemicus．We cultivated the deleted strains and the wide type in CDM2， and found the cell concentration of AgntR4 was obviously lower than wide type in the early growth stage．According to detecting the concentration of AgntR4 and the wide type in the early period(1-1 2 h)，we found that AgntR4 grown obviously slower than the wide type in the exponential phase．To analyze the influence of different carbohydrate source to AgntR4， we cultivated A gntR4 and the wide type in liquid and solid medium with different carbohydrate source．We found the growth difference Was most apparent in liquid medium with only glucose when cultiva