切断单侧角膜神经对于双侧角膜上皮屏障功能的影响-眼科学专业论文.pdf.docxVIP

中文摘要环钻对侧组角膜的P物质及其受体NK一1R的mRNA表达水平在处理后升高，同时 中文摘要 环钻对侧组角膜的P物质及其受体NK一1R的mRNA表达水平在处理后升高，同时 炎症因子IL一1 13、TNF．Q、VEGF-a的mRNA表达水平都有所升高。划“×”组 的ZO．1和E．cadherin的分布在处理后第l天排列不整齐，P物质、NK．1R、IL．1 B、 VEGF—a的mRNA表达量在处理后第1天表达上调，在第7、14天与对照组相比无 显著变化。划“×”处理眼的对侧组在上述的观察指标中与对照组相比均无明显 变化。 结论：切断单侧的角膜神经后，出现双侧角膜上皮屏障功能的破坏。同时我 们发现，角膜神经破坏后双侧角膜中P物质、NK一1R和炎症因子的表达量也上调。 切断角膜神经引起的角膜上皮屏障功能破坏，可能由于P物质及炎症因子表达上 调所致。 关键词：角膜神经；上皮屏障；炎症 II 万方数据 ABSTRACTABSTRACT ABSTRACT ABSTRACT Purpose：Normal corneal epithelial barrier plays important role in maintaining corneal homeostasis and protect cornea from invastion．At present we already know that ocular trauma，inflammation，metabolic disorders could change the distribution and expression of corneal superficial epithelium junction，and could lead to dysfunction of comeal epithelial barrier function．Corneal epithelial barrier dysfunction is the pathological basis of various ocular surface diseases．It has been confirmed that corneal nerves could communicate and contact with corneal epithelium． Our study intended to explore the effect of bilateral corneal epithelial barrier function after severing unilateral corneal nerves，and the possible mechanism of this phenomenon． Method：The normal wistar rats were randomly divided into three groups by different methods：control group，trephine treated group，scratched II×”group，each group had 30 rats in it．Trephine treated group means we used 3．00 mrs diameter core trephine cut from corneal epithelium to stromal，which was about two．thirds of corneal stromal layer deep，the contralateral eye didn’t do any test．We scratched two perpendicular lines about 3．00 mnl diameter with blade paddle the cornea．The contralateral eye didn’t do any test．Then we assessed the ocular surface of experimental rats on day 1，day 7，day 1 4，did the following clinical index detection：1． corneal sensation，2．sodium fluorescein staining，3．tear secretion measurement．We did hematoxylin-eosin(HE)staining to observe the morphological changes in comeal epithelium，a