一株降解石蜡菌株的分离鉴定及其表面活性剂的研究对-微生物学专业毕业论文.docxVIP

ABSTRACTThe ABSTRACT The wax content of oil field well is higll，and its molecules usually consist of long alkane chains．This makes serious Wax precipitation that usually needs some methods to dear．The common paraffin removal me嬲ures include hot water,oil or chemical remover and inhibitor． However,these methods are often expensive and labor consuming．Chemical paraffin inhibitor has some negative influences bom on the oil quality and workers health．In addition，clearing wax with hot water often results in block up，while hot oil breaks the oil well． In this study,An alkane··degrading stain was isolated from oil··water mixture of the surface in Qinghai oil well．This strain was isolated and characterized by 1 6S rDNA．The system envolution WaS also made,and its biochemical character and optimal culture conditions were investigated．Then the culture conditions and the Wax-degrading conditions were optimized．A hi曲ly efficient wax—degrading strain Y7 was acquired by ARTP mutation． Moreover,the biosurfactant produced by Y7 WaS studied．Wax degradation experiment showed that this strain was able to degrade wax and emulsification phenomenon occurred． Then the biosurfactant produced by this strain Was also studied．Results ale as following： (1)Biochemical character and 1 6S rDNA sequence results show that the strain isolated from soil．water mixture is belonging to Alcanivorax dieselolei：G。and no spore． (2)The optimal culture conditions are：the volume of liquid 60 mL／250mL，initial pH 7．0，temperature 33℃，1 50 rpm，culture time 7 d，NaCI 30 g／L，K2HP04 2 g／L，MgS04 0．4 g／L， albumen powder as nitrogen source，vegetable oil as carbon source，The highest yield reaches3．688x 1 0¨／rnL，increasing 1 05 times． (3)The optimal Wax—degrading conditions are：the initial concentration of paraffin：209／L，the initial concentration of glucose：1 2班，initial pH 8．0，temperature 33 ℃，1 80 rpm，culture III 万方数据 time time 9 d，NaCI 1 5 g／L，KaHP04 3 g／L，FeS04 0．05∥L，MgS04 0．8 g／L，The highest wax failing rate：58．87％．