玉米中QM同源基因的克隆及其差异表达分析.PDFVIP

作物学报 ACTA AGRONOMICA SINICA 2009, 35(8): 1439− 1444 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.01439 玉米中QM 同源基因的克隆及其差异表达分析 王会伟 李洪杰 朱振东 武小菲 王晓鸣* / , 100081 : cDNA-AFLP 5′ RACE Ht2 QM(L10) (ZmQM) cDNA 967 bp, 738 bp245 ZmQM , 27.78 kD, 10.69, C N- ZmQM l3 QM , 66%~92%RT-PCR , (Exserohilum turcicum) 1 12 h , Ht2 ZmQM , ZmQM Ht2 1 : QM ; L10; ZmQM ; Ht2 ; Exserohilum turcicum Cloning and Differential Expression of QM-Like Protein Homologue from Maize * WANG Hui-Wei, LI Hong-Jie, ZHU Zhen-Dong, WU Xiao-Fei, and WANG Xiao-Ming Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing 100081, China Abstract: A full-length QM-like cDNA (designated ZmQM) was cloned from maize (Zea mays L.) leaf tissues using cDNA am- plified fragment length polymorphism (cDNA-AFLP) and rapid amplification of cDNA ends (RACE) techniques. The expression of ZmQM was examined in leaves of the Ht2 isogenic lines Huangzaosi and HuangzaosiHt2 carrying gene Ht2 for resistance to northern corn leaf blight after inoculation with race 1 of Exserohilum turcicum (Pass.) Leonard et Suggs. Gene ZmQM contains an open reading frame 738 bp in length, which encodes 245 amino acids with a predicted molecular weight of 27.78 kD and an isoelectric point of 10.69. Scanning PROSITE motifs indicated that the amino acid sequence of ZmQM protein in