灰岩皱叶报春和滇北球花报春在热锻炼和热胁迫下-华南农业大学学报.PDFVIP

华南农业大学学报 Vol. 31 , No.3 第 31 卷第3 期 Joumal of South China Agricultural University Jul. 2010 2010 年7 月 灰岩皱叶报春和滇北球花报春在热锻炼和 热胁迫下叶肉细胞超微结构的差异 胡伟娟，张启翔，潘会堂，董玲玲 (北京林业大学园林学院，北京，100086 ) 摘要:为研究报春花属 Primula 植物对高温的响应机制，从超微结构的角度探讨热锻炼提高耐热性及不同品种耐热 性差别的原因，利用透射电镜的方法，对耐热种灰岩皱叶报春 P. forrestii 和不耐热种模北球花报春P. dent比ulωe ssp. sinodenticulata 进行了热锻炼(38 c , 12 h) 和热胁迫(42 c )条件下细胞超微结构的观察.结果表明，热锻炼提 高了核膜的热稳定性，延缓热胁迫对叶肉细胞超微结构的破坏，从而可提高细胞的耐热性.耐热种和不耐热种在热 锻炼与热胁迫过程中的超微结构变化过程基本相同，只是被破坏的时间有差异;叶肉细胞中叶绿体比线粒体对高 温敏感，在热胁迫过程中，存在线粒体数量增多的现象，推测是报春属植物对逆境的积极响应对策. 关键词:报春花属;热锻炼;热胁迫;超微结构 中图分类号:5682. 15;Q248 文献标识码:A 文章编号: 1001-411X(2010 )03-∞43-04 DifTerence in U1trru写tructure of MesophyU Ceu Between Two Primula Species with DifTerent Thelmotolerance Under Heat Åcclimation and Heat Stress HU Wei-juan , ZHANG Qi-xiang , PAN Hui-tang , DONG Ling-ling (College of Landscape Architecture ,Beijing Forestry University ,Beijing 1侧83 ， China) Abstract: To study the response mechanism of Primula to high temperature and find the reasons for the enhancement of heat resistance after heat acclimation and the difference in heat resistance between differ- ent species from the perspective of the ultrastructure ,the ultrastructure of mesophyll cell in heat-resistant species (Primulα forrestii) and non-heat-resistant species (P. denticulate ssp. sinodenticulatα) were inves- tigated under the heat acclimation (38 c , 12 h) and heat stress (42 C) using the method of transmis- sion electron microscopy. The results showed that heat acclimation improved thermotolerance o