新的产弹性蛋白酶菌株的筛选与鉴定-微生物学报.PDF

微生物学通报 SEP 20, 2009, 36(9): 1345~1349 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 新的产弹性蛋白酶菌株的筛选与鉴定 * 刘双发 安德荣 苟丽霞 李 娜 ( 712100) 摘 要: 本研究从秸秆中分离得到一株弹性蛋白酶高产菌, 并对该菌株进行鉴定, 以期为实现其 工业化生产提供理论依据。采用酪蛋白(脱脂奶粉)进行初筛后, 以弹性蛋白(牛筋)进行复筛, 并对 筛选结果进行检验, 然后对所得菌株结合形态、生理生化特性和16S rRNA基因序列进行分类鉴定, 得到一株弹性蛋白酶高产菌株LSF-97 。结果显示, 菌株LSF-97 与短小芽孢杆菌同源率达到 100%, 形态及生理生化特征与模式菌也显示高度一致性, 故将其确定为短小芽孢杆菌。 关键词: 芽孢杆菌, 蛋白酶, 筛选, 鉴定 Screening and Identification of a New Elastase-producing Strain * LIU Shuang-Fa AN De-Rong GOU Li-Xia LI Na (College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A F University, Yangling, Shaanxi 712100, China) Abstract: The study provide a theoretical basis for the industrial production of a elastase-high-yield strain which was isolated from the straw, and the selected strain was identified. The screening strategy included casein (skim milk) plate selecting and elastin (beef tendon) re-screening. And then, the morphological, physiological and biochemical characteristics as well as 16S rRNA sequence homology of the selected strain were studied. Finally, the strain LSF-97 which had a excellent decomposing ability to beef tendon was ob- tained. The results showed that the strain LSF-97 is relative to the Bacillus pumilus with 100 % similar in sequence under