耐旱基因siupf3的生物信息学和功能分析-生物学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 耐旱基因SiUPF3 耐旱基因SiUPF3的生物信息学和功能分析 The Bioinformatic and Physiological Analysis of Drought—tolerant Gene SiUPF3 Ab stract Drought is one of the most significant factors which affect plant growth in the natural environment．In China，the arid and semi—arid areas are as high as 30％，which badly affects the production of grain and other economic crops in China．Meanwhile，we also found that in the western regions of China some minor-coarse grain crops all the year round can grow normally under the stress of drought conditions and its production barely affected by drought．Setaria italica is one of the minor·coarse grain crops mentioned above．As a C4 plant，Setaria italic have resistance to drought，disease and beating down．In this study we selected‘Jingu 34’as the object and material and select genes which can response to drought significantly from gene expression profile．Finally，we got a drought—related gene which encodes Upt3 protein and named it SiUPF3． The related literature shows that Upf3 proteins involved in the Nonsense．mediated decay pathway which iS an important mRNA degradation pathways in eukaryotic．So far．NMD researches in plant lag far behind those in animals and yeast and the methods of NMD research in plant are also relatively backward．To solve this problem，this article will give a relatively comprehensive molecular mechanism of NMD in the review． In order to further explore the function of drought．tolerant gene SiUPF3 involved in NMD way，we processed bioinformatics analysis and gene expression analysis(dealed with drought and abscisic acid)、then we cloned SiUPF3 and constructed the transient expression vector with green fluorescent protein tag and the plant expression vector to carry the SiUPF3 subcellular localiza -tion and get transgenic strains ofNicotiana by transgenic method respectively．Finally we found that SiUPF3 mainly exists in plant cell nucleus and obtained three overexpressed strains named S 1． S2．S3 which call be used for phsiologi