反复冻融对速冻水饺菌相变化影响实习.pdfVIP

现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.5 反复冻融对速冻水饺菌相变化的影响 韩齐，孙清秀，孙方达，郑冬梅，孔保华 （东北农业大学食品学院，黑龙江哈尔滨150030 ） 摘要：本文同时采用PCR-DGGE （聚合酶链式反应-变性梯度凝胶电泳）方法和传统平板培养法两种方法研究了反复冻融条件下 造成的温度波动对速冻水饺中微生物数量及其种类多样性的影响。并向水饺中人为添加金黄色葡萄球菌，分析反复冻融条件下金黄色 葡萄球菌数量的动态变化及其对水饺中菌相的影响。研究结果表明随着反复冻融次数的增加，水饺中的微生物数量均呈显著增加趋势 （P0.05 ），人工接种金黄色葡萄球菌处理组水饺中金黄色葡萄球菌数量从速冻后的3.49 lgCFU/g 增加至5.07 lgCFU/g，已经达到了 5 可导致葡萄球菌属中毒的水平（10 CFU/g ）。PCR-DGGE 结果表明，乳酸菌、假单胞菌和热杀索丝菌为反复冻融过程水饺中的优势菌 株，葡萄球菌属在后期数量较多成为反复冻融后期的优势菌株，与传统平凡培养方法结果基本一致。 关键词：水饺；聚合酶链式反应-变性梯度凝胶电泳；多样性；优势菌群；金黄色葡萄球菌 文章篇号：1673-9078(2015)5-206-211 DOI: 10.13982/j.mfst.1673-9078.2015.5.033 Effect of Multiple Freeze-thaw Cycles on the Microflora of Quick-frozen Dumplings HAN Qi, SUN Qing-xiu, SUN Fang-da, ZHENG Dong-mei, KONG Bao-hua (College of Food Science, Northeast Agricultural University, Harbin 150030, China) Abstract: The effect of temperature fluctuations caused by repeated freeze-thaw cycles on the number and diversity of microorganisms in quick-frozen dumplings was investigated usingpolymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and traditional plate culture methods. The dumpling fillers were inoculated with Staphylococcus aureus to analyze the dynamic changes in S. aureus itself and its influence on the dumpling microflora. The results showed that the quantity and diversity of microorganisms in the dumplings increased significantly (p 0.05) with increasing freeze-thaw cycles. At the last cycle, S. aureus count in the inoculated dumplings increased from 3.49 to 5.07 log10 CFU/g, which reached the minimum count for Staphylococcal toxicity, of 105 CFU/g. PCR-DGGE showed that lactic acid b