转2mGepsps基因水稻与亲本水稻的种子蛋白-中国粮油学报.DOC

PAGE \* MERGEFORMAT PAGE \* MERGEFORMAT 11 转2mG2-epsps基因水稻种子蛋白质非预期变异分析 李燕燕 赵 艳 （浙江工商大学食品与生物工程学院，杭州 310018) 摘 要 以转2mG2-epsps基因抗草甘膦水稻为材料，根据实质等同性原则，分析转基因（genetically modified, GM)水稻种子可溶性蛋白含量和组分的非预期变异。结果表明：（1）与非转基因亲本相比，GM水稻种子的总可溶性蛋白含量极显著降低，主要受谷蛋白含量变异影响。但清、球、醇、谷四种蛋白含量的最高变异幅度（9.12%）低于P1和P2两亲本之间的最大差异幅度（14.67%）。说明GM水稻种子的可溶性蛋白含量非预期变异在安全范围内，但营养品质可能与亲本品系存在差异。（2）SDS分析发现，GM水稻株系T12和T22分别有12和14个蛋白谱带发生了非预期变异，涉及四种可溶性蛋白的组分，多数表现为含量变化。突出的是，2个GM水稻株系中均新增加了41 ku和56 ku谷蛋白组分，需要进一步通过毒理学分析评价其食品安全性。 关键词 转基因水稻; 种子可溶性蛋白; 实质等同性; 非预期变异 中图分类号：S188 文献标识码：A Analysis of Protein Unintended Variation of Transgenic Rice Seeds With 2mG2-epsps Gene Li Yanyan Zhao Yan ( College of Food Science and Biotechnology, Zhejiang Gongshang University，Hangzhou 310018 ) Abstract The unintended variation of soluble protein contents and components of genetically modified(GM) rice lines were analyzed, using glyphosate-resistant GM rice lines harboring 2mG2-epsps gene as materials, according to the principle of substantial equivalence. The results showed: (1) Compared to the non-transgenic parent lines, the total soluble protein 基金项目：农业部转基因生物新品种培育重大专项（2011ZX08010-003）；国家自然科学基金。 作者简介：李燕燕，女，1987年出生，硕士，生物工程专业。 通讯作者：赵 艳，女，1970年出生，教授，硕士生导师，植物生化与分子生物学。 Tel： 0571E-mail：yanzhao9918@。 contents of GM rice seeds decreased very significantly, which are mainly affected by the glutenin content variation. While the maximum content variation range (9.12%) from four kinds of proteins including albumin,?globulin, prolamin,?glutenin, is lower than the variation difference (14.67%) of the two parent varieties P1 and P2. This indicated that the unintended variation of soluble protein contents of GM rice lines is among the safety range, but the nutritional quality of GM rice seeds might be different with their parent varieties. (2) SDSanalysis revealed that there are 12 and 14 unintended variation protein bands in the respective GM rice line T12 and T22, concerning four kinds of so