绿海龟外周血细胞显微结构与分类-Seaturtleorg.PDFVIP

第 28 卷 第 3 期 广东海洋大学学报 Vol.28 No.3 2008 年 6 月 Journal of Guangdong Ocean University Jun. 2008 绿海龟外周血细胞显微结构与分类 李长玲，曹伏君，黄翔鹄，刘楚吾，麦哲文 （广东海洋大学水产学院，广东 湛江 524025） 摘 要：对绿海龟(Chelonia mydas)外周血细胞的进行显微观察。结果表明，经 Wright’s 染液染色，可鉴定出红血细 胞、单核细胞、淋巴细胞、血栓细胞、嗜酸性粒细胞、嗜碱性粒细胞、嗜中性粒细胞等七种血细胞。其中红血细胞 数量多，长椭圆形，具圆核。白细胞数量少，多为圆形，分无颗粒型白细胞和颗粒型白细胞两种。无颗粒型白细胞 包括单核细胞和淋巴细胞，细胞质中无特殊颗粒；颗粒型白细胞包括嗜酸性粒细胞、嗜碱性粒细胞和嗜中性粒细胞， 细胞质中具有特殊颗粒。在白血细胞中，血栓细胞体积最小，单核细胞体积最大；在数量上，血栓细胞最多，淋巴 细胞次之，单核细胞和嗜碱性粒细胞则较少。绿海龟的淋巴细胞可分为大淋巴细胞和小淋巴细胞两种类型。在外周 血细胞中可观察到分解、解体状态的红血细胞，还可观察到少量未成熟的红血细胞和嗜中性粒细胞，偶尔可见正在 分裂的红血细胞，提示红血细胞也可在外周血直接分裂。 关键词：绿海龟；血细胞；显微结构 + 中图分类号：Q959.6 32 文献标志码：A 文章编号：1673-9159（2008）03-0024-05 Microstructure and Identification of Peripheral Blood in Chelonia mydas LI Chang-ling, CAO Fu-jun, HUANG Xiang-hu, LIU Chu-wu, MAI Zhe-wen (Fisheries College, Guangdong Ocean University, Zhanjiang 524025,China) Abstract: The morphology of peripheral blood cells of the Chelonia mydas was studied. On the stained smears, seven major types of cells are recognized: erythrocyte, thrombocyte, lymphocyte, monocyte, neutrophil, eosinophil and basophil. Among these cells, erythrocyte is the most abundant and has round nucleus. Leucocytes are divided into two types: granulocytes and non-granulocytes. Non-granulocytes include lymphocyte and monocyte; granulocytes include neutrophil, eosinophil and basophil. In size, thrombocyte is the smallest among leucocytes, and monocyte is the largest; in quantity, thrombocyte is the largest, lymphocyte the second, then monocyte and basophil. Lymphocytes can