基因治疗载体及其基因转移技术的关键问题与研究现状.PDF

1 1 Vol. 1,No. 1 1997 12 Life Science Research Dec. 1997 唐冬生　　夏家辉 ( , ,410078) 目前使用的基因治疗载体及其基因转移技术中还没有 一种能用 临床 并永久有效的基因转移技术. 该文分析了直接体内转移、间接体内转移及其非载体 法、病毒载体法、非病毒性生物载体法等基因治疗转移技术存在的 一些关键问题, 并 探讨了各问题的解决办法或研究策略以及基因治疗载体研究的发展方向. ,, 394 R The Problems and Progress of The Vectors and Transgene Techniques for Gene Therapy Tang Dongsheng, ia Jiahui (National Laboratory of Medical Genetics,Hunan Medical University,Changsha 410078,Hunan,PRC) AbstractNone of the currently available vectors and transgene techniques for gene therapy is cliniclly useful and permanent cure.The problems of the vectors and transgene techniques such as in vivo transgene techniques, ex vivo transgene techniques, non- vector methods, virus vector methods non, - virus biologic vector methods etc have been analyzed T he, . . methods solving these problems,the research strategy and developing tendency of vectors for gene therapy have been discussed. Key wordsgene therapy vector gene transfer, , 80 ,, 90 (adenosine deaminase,ADA) ., AIDS.,21 ,,. , .(in vivo) (ex vivo) . : Smith Kline Beecham : 1997- 10- 23. : , , 33 , , ; , , 61, . 1 : 17 . , , ,. Francis Collins , [ 1] . , . 1 . : : DNA , ,; :, , ; : ,; : , . , , , ,, ., . 1) DNA .,DNA , ,, . 2) . DNA DNA , [2] . 3) .DNA , [3] , . [4] 4) . DNA, . DNA .DNA , , . 5) . DNA