益生菌细菌素抑菌活性探究.pdf

Medical Diagnosis 医学诊断, 2015, 5, 6-11 Published Online March 2015 in Hans. /journal/md /10.12677/md.2015.51002 A Study on Bacteriostatic Activity of Bacteriocins Produced by Probiotic Bacteria 1 2 1 1 1* Lan Wang , Yan Zhou , Biting Wu , Haiyan Li , Jimei Du 1 School of Laboratory Medicine, Wenzhou Medical University, Wenzhou Zhejiang 2 Demonstration Center of Biological Experimental Teaching, Wenzhou Medical University, Wenzhou Zhejiang * Email: hanazhou@126.com, djm@ th th th Received: Mar. 17 , 2015; accepted: Mar. 20 , 2015; published: Mar. 26 , 2015 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Object: To reveal the bacteriostatic activity of bacteriocins produced by human intestinal probio- tics. Methods: Intestinal probiotics were separated from excrement of healthy people and identi- fied by routine method and PCR. Bacteriocins were refined as Yang’s way and strains that could produce broad-spectrum efficient bacteriocins were selected by using 96-well plate method. Bac- teriostatic activity of every selected bacteriocin was detected on S. aureus, S. pneumoniae, E. coli, K. pneumonia, P. vulgaris, S. typhi, S. flexneri, P. aeruginosa, A. baumanni and C. albicans by 96-well plate method respectively. Results: 32 strains of intestinal probiotics were separated, including 4 bifidobacteria (B), 6 lactobacillus (L), and 22 enterococcus (E). B3, B4, L5, E12, E15, E18 were broad-spectrum efficient