高效液相色谱法同时测定鲜水果中乙萘酚和联苯醚等中国农学通报.PDF

中国农学通报 2014,30(6):290-294 Chinese Agricultural Science Bulletin 高效液相色谱法同时测定鲜水果中乙萘酚和 联苯醚等4 种防腐保鲜剂残留量 黄秀丽，曾宪远，付丽敏，唐丽娜，陈清清，奉夏平 （广东省惠州市质量计量监督检测所，广东惠州516003） 摘 要：为了更好地对市场上出售的鲜水果的质量安全进行监管，建立了同时测定鲜水果中4 种防腐保 鲜剂包括对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、乙萘酚和联苯醚残留量的高效液相色谱法。样品经乙 醚提取后，活性炭柱净化。C18反相色谱柱分离，流动相为甲醇:水（55:45 ，磷酸调节pH≈3），二极管阵列 检测器，检测波长为208 nm 。结果表明：各防腐保鲜剂的回收率在85.4%~104.7% ，相对标准偏差为 1.10%~4.75% 。该方法简便、快捷，且重复性好，可满足鲜水果中对羟基苯甲酸甲酯、对羟基苯甲酸乙 酯、乙萘酚和联苯醚残留量的测定。 关键词：高效液相色谱；鲜水果；防腐保鲜剂；残留量 中图分类号：O658 文献标志码：A 论文编号：2013-1155 Determination of the Residue Amout of 4 Preservatives in Fresh Fruit by High Performance Liquid Chromatography Huang Xiuli, Zeng Xianyuan, Fu Limin, Tang Lina, Chen Qingqing, Feng Xiaping (HuizhouQualityandMeasuringSupervisionTestingInstitute, Huizhou Guangdong 516003) Abstract: A method was established to detect 4 kinds of fruit preservatives including p-methyl hydroxybenzoate, p-ethyl hydroxybenzoate, ethyl naphthol and diphenyl ether by high performance liquid chromatography (HPLC) to supervise the quality safety of fresh fruit. The samples were extracted by ether and purified by activated carbon column. For the determination, reversed phase C18 column was used and mobile phase was 55% CH OH-45% H O (pH ≈3). A Diode array detector was used at 208 nm. Standard addition 3 2 recovery ranged from 85.4% to 104.7% with relative standard deviation(RSD) from 1.10% to 4.75% . The method was suitable for the determination of p-methyl hydroxybenzoate, p-ethyl hydroxybenzoate, ethyl naphthol and diphenyl ether residues in fresh fruits with high sensitivity and selectivity. Key words: h