植物生理学与生物化学国家重点实验室2010年报.PDFVIP

植物生理学与生物化学国家重点实验室 2010年报 The Plant Cell, Vol. 22: 2336–2352, July 2010, ã 2010 American Society of Plant Biologists DNA Replication Factor C1 Mediates Genomic Stability and Transcriptional Gene Silencing in Arabidopsis W OA Qian Liu,a,1 Junguo Wang,a,1 Daisuke Miki,b,c Ran Xia,a Wenxiang Yu,a Junna He,a Zhimin Zheng,b,c Jian-Kang Zhu,b,c,d and Zhizhong Gonga,d,e,2 a State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China b Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, California 92521 c Center for Plant Stress Genomics and Technology, 4700 King Abdullah University of Science and Technology, Thuwal 23955- 6900, Kingdom of Saudi Arabia d China Agricultural University–University of California, Riverside Center for Biological Sciences and Biotechnology, Beijing 100193, China e National Center for Plant Gene Research, Beijing 100193, China Genetic screening identified a suppressor of ros1-1, a mutant of REPRESSOR OF SILENCING1 (ROS1; encoding a DNA demethylation protein). The suppressor is a mutation in the gene encoding the largest subunit of replication factor C (RFC1). This mutation of RFC1 reactivates the unlinked 35S-NPTII transgene, which is silenced in ros1 and also increases expression of the pericentromeric Athila retrotransposons named transcriptional silent information in a DNA methylation- independent manner. rfc1 is more sensitive than the wild type to the DNA-damaging agent methylmethane sulphonate and to the DNA inter- and intra- cross-lin