高温胁迫与超声刺激促进微藻油脂积累的过程及机理环境工程专业论文.docxVIP

山东大学博士学位论文3．2．4 山东大学博士学位论文 3．2．4 高温对微藻蛋白的影响 ．．5 1 3．3 小结 5 l 第四章 超声刺激微藻积累油脂 53 4．1 超声对四尾栅藻的影响 54 4．1．1 实验材料与方法 ．．54 4．1．2 结果与讨论 ．．58 4．1．3 小结 ．．64 4．2 超声对多变鱼腥藻的影响 65 4．2．1 实验材料与方法 ．．65 4．2．2 结果与讨论 ．．69 4．2．3 小结 ．．79 第五章 研究结论与展望 8 l 5．1 主要结论 8 l 5．1．1 微藻生长条件优化及新型优化方案对微藻生长的促进 ．．8l 5．1．2 高温胁迫促进微藻油脂积累 ．．82 5．1．3 超声刺激促进微藻油脂积累 ．．82 5．1．4 高温与超声促进微藻油脂积累的机理 ．．83 5．2 研究创新点 84 5．3 存在问题与展望 84 参考文献 87 致谢 ．．103 攻读博士学位期间的科研情况 1 05 Ⅱ 万方数据 山东大学博士学位论文CoNTENT 山东大学博士学位论文 CoNTENT Abstract ．．V ABSTRACT ．．IX Symbol description XIII Chapter l Introduction ··1 1．1 Research background ．．1 1．2 Microalgae overview 2 1．2．1 Characteristics ofmicroalgae ．2 1．2．2 Cultivation ofmicroalgae 3 1．2．3 Components ofmicroalgae ．7 1．3 Methods of improving microalgae biomass production ．．1 0 1．3．1 Isolation of new algae and development of new photobioreactor 1 0 1．3．2 Optimization of algae growth condition 11 1．4 Measures ofenhancing microalgae lipid accumulation ． ． 12 1．4．1．7rreatment ofnutrients stress 12 ．1j4．2 Treatment ofhigh temperature and ultrasound ．．15 1．5 Contents and innovation ofthe research ．16 1．5．1 Research objectives ··1 6 1．5．2 Research contents ．．1 7 1．5．3 Research innovation ．1’7 Chapter 2 New schemes for optimization of microalgae growth conditions ．．1 9 2．1 Materials and methods ．．20 2．1．1 Microalgae species，culture medium and experimental apparatus 20 2．1．2 Optimization of microalgae growth conditions ．22 2．1．3 Analysis methods ．．23 2．2 Results and discussion ．．25 2．2．1 Efrects of initial cell density 25 2．2．2 E疏cts of light intensity ．26 2．2．3 Effects ofaeration rate 28 2．2．4 New schemes for optimization ofgrowth conditions ．30 2．2．5 Fatty acid compositions and properties ．。33 2．3 Conclusions 35 Chapter 3 High—temperature stress for microalgae lipid accumulation． 37 3．1 Materials and methods ．．38 3．1．1 Microalgae species，culture medium and experimental apparatus 38 3．1．2 E行ects ofhigh．temperature stress lipid content ．39 3．1．3 Analysis methods ．．40 3．2 Results and discussio