k562细胞规模化培养及硒酸多糖诱导其凋亡的研究食品工程专业论文.docxVIP

ABSTRACTIn ABSTRACT In thjs study，t11e baSic culture conditions of cl们11ic myelogenous leul(emia(CML) K562 cells were researched by bio-reactor．Simultalleously， t11e apoptosis of K562 cells inducedby selenic acid canila萨polysacch撕de were also studied．The cell mo叫ological chal．acteristics were obserVed by HE a11d Hoechst 33342／PI．The cell cycle剐陌est aIld mitochondrial membrane potential(△、王，m)were aSsessed by Flow Cytome臼呵(FCM)．The expression of印optosis—related protein and gene were allalyzed by inunuIlonuorescence a11d Real．time PCR． Firstly’tlle basic culture conditions of bio-reactor includi