堆肥中βglucosidase家族微生物群落与纤维素降解的相关性研究生物学微生物学专业论文.docxVIP

CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese． ． ．．1 Abstract in English ． ． ． ．．III 1．Introduction 1 1．1 Summary of agricultural solid wastes ． ． ．．．1 1．1．1 Current situation ofagricultural waste ． ．．1 1．1．2 Agricultural waste treatment technology 2 1．1．3 The meaning of agricultural waste utilization． ．4 1．2 Overview of composting 4 1．2．1 The concept and principle ofcompost ． ． ．．5 1．2．2 Effect factors ofcomposting ．．． ．．．．． ．． ．．5 1．2．3 Diversity ofcellulose degrading bacteria ．．．． ．6 1．3 Cellulose degrading of composting ． ．7 1．3．1 Overview ofCellulose 8 1．3．2 Diversity ofcellulose degrading bacteria ． ． 8 1．3．3 Cellulose degrading enzymatic characteristics． ． ． 8 1．3．4 Overview ofl3-glucosidase ． ．． ．． ．．． 8 1．4 The research methods ofmicroorganism 8 1．4．1 The principles ofmicrobial degradation ． 9 1．4．2 Composting microbial community and dynamic 9 1．4．3 Research methods ofmicrobial ． ． ． ．．9 1．4．4 Microbial genomics ．9 1．5 Proposed research idea ．10 1．6 The purpose and meaning of research ． ．．．． ． 10 1．7 Technical route ．． ．．10 2．Materials and Methods ．．1 3 2．1 Materials ．13 2．1．1 Test site ． ．． ． ． 1 3 2．1．2 Sampling 13 2．1．3 Instruments and reagents ．． ． 13 2．2 Methods．． ． ．．． ．．14 2．2．1 Temperature measurement ． ． ．．14 2．2．2 Determination of enzyme activity ． ．14 2．2．3 Determination of cellulose content ．． ．14 2．2．4 Extraction and purification oftotal DNA ． ． ．22 2．2．5 Amplification oftarget gene ·24 3．The results and analysis ．25 万方数据 3．1 3．1 Changes in the physicochemical properties ‘‘。 3．1．1 Changes in physical properties during composting “ 3．1．2 The changes ofchemical properties during composting ’’二J 3．2p