低硒日粮对肉鸡免疫器官中硒蛋白与细胞因子表达的影响兽医学基础兽医学专业论文.docxVIP

CoNTENTSAbstract CoNTENTS Abstract in I：：hinese ． l Abstract in English ．．． ． ． ． ． ． ． ． ． ． ． ． ． ． ． ． ．． ． ． ． ． ．． III 1 Intruduction ．．1 1．1 Selenium and Selenoproteins ． ． ． ．．1 1．1．1 The classification， function and location of seleno口roteins ．．1 1．1．2 The regulation of selenium to selenoprotein in tissues 4 1．2 The effects of selenium defiency on immune organs ．．4 1．2．1 The effects of Se defiency on immune organs morphological structures ．4 1．2．2 The effects of selenium defiency on immune functions ．．5 】1．3 Purpose and significance ofthe study．． ．．． ．．． ． ． ． ． ． ． ． ． ． ． ．． ．．6 2 Materials and methods ．7 ：!．1 Main instruments and reagents． ． ．7 2．1．1 Main instruments ．．7 2．1．2 Main reagents ．7 2．2 Experimental animal and the ED broilerc chickens modelling ．．8 2．2．1 Experimental animal group and feeding ．8 2．2．2 Tissue samples collection and handling methods 9 2．3 Detection indexes and method of experiment ．9 2．3．1 Observation ofhistopathology 9 2．3．2 Total RNA extraction in the immune tissues and eDNA synthesis ．9 2．3．3 23 kinds of seleniumprotein mRNA expression in immune tissues ．11 2．3．4 Detectionthe mRNA Ievels ofcytokines in immune organs ．．13 2．4 The experimental data statistical analysis 1：； 3 Results and alialysis．．．．．．．．．．．．．．．． ．．． ． ．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ． ． ．． ． ．．14 3．1 Pathological observation ．．．．． ． ．．． ． ．．．． ．． ． ． ． ． ． ． ． ． ． ． ．． ．14 3．1．1 The rsult ofobserval[ion in spleen ．．14 3．1．2 The rsult ofobservation in thymus ．15 3．1．3 The rsult ofobservation in bursa ofFabricus 17 3．2 The results of Se deficiency Sels gene expressions in immune tissues ．18 3．2．1 The results of Se deficiency Sels gene expressions in immune tissues l 8 3．2．2 The results of23 Sels expression patterns in broiler immune organs ．29 3．3 The effects of Se def