HPLC测定不同产地罗勒中芦丁和槲皮素的含量.PDFVIP

HPLC 测定不同产地罗勒中芦丁和槲皮素的含量 陈良1 1 2 1 ，徐晓琴 ，凯撒·苏来曼 ，姜林 (1.新疆医科大学附属中医医院，乌鲁木齐 830000 ；2.新疆维吾尔自治区民族药物研究所， 乌鲁木齐 830002) 摘要：目的 建立罗勒中芦丁和槲皮素的含量测定方法，并对不同产地药材进行含量测定。方法 采用高效液相色谱法 将罗勒中芦丁和槲皮素进行测定；芦丁的流动相为乙腈-0.4%磷酸(18 ∶82) ，槲皮素的流动相为乙腈-0.4%磷酸(32 ∶68) ； 1 检测波长均为360 nm ；流速均为1.0 mL·min ；柱温均为30 ℃。结果 芦丁的线性范围为4.95~24.75 g(r ＝0.999 7) ，平 均回收率为 100.7%，RSD=1.62%(n=6) 。槲皮素的线性范围为1.792~8.064 g(r=0.999 7) ，平均回收率为98.3% ，RSD 为 0.94%(n=6) 。不同产地罗勒药材中芦丁和槲皮素的含量分别在0.39~0.71 mg·g1 和0.29~0.58 mg·g1 之间。结论 本法专 属性强，重复性好，结果准确可靠，可作为罗勒的质量控制方法。 关键词：罗勒；芦丁；槲皮素；高效液相色谱法 中图分类号：R927.2 文献标志码：B 文章编号：1007-7693(2011)04-0344-03 Determination of Rutin and Quercetin in Ocimi Herba from Different Places by HPLC 1 1 2 1 CHEN Liang , XU Xiaoqin , KAISA Suerman , JIANG Lin (1.Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University, Urumqi 830000, China; 2.Xinjiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, China) ABSTRACT: OBJECTIVE To establish a method for determination of the contents of rutin and quercetin in Ocimi Herba, and to determine the contents of rutin and quercetin in Ocimi Herba from different places. METHODS The contents of rutin and quercetin in Ocimi Herba were determined by HPLC. The rutin was determined by using mobile phase consisted of acetonitrile-0.4% phosphoric acid solvent(18 ∶82), the quercetin was determined by using mobile phase consisted of acetonitrile-0.4% phosphoric acid solvent (32 ∶68). All the UV wavelength was 360 nm, the flow rate was 1.0 mL·min1 and the column temperature was 30 ℃. RESULTS The linear range of rutin was 4.9524.750 μg(r=0.999 7) with a mean recovery of 100.7% and RSD 1.62%(n=6). The linear range