番茄spwrky6转录因子的抗病功能研究生物学专业论文.docxVIP

番茄SpWRKY6转录因子的抗病功能研究Functional 番茄SpWRKY6转录因子的抗病功能研究 Functional Analysis of Tomato SpWRKY6 Transcription Factor in Disease Resistance Ab stract Late blight caused by the oomycete pathogen Phytophthora infestans，which causes a major threat to tomato production．and it always led to severe economic lOSS．The predominant method to control for late blight is through frequent use of fungicides and traditional breeding method．However，fungicides application is costly and has a negative impact on environmental safety and human health．强e traditional breeding method cultivates resistant varieties，possessing some drawbacks such as cycle long．As the development of biotechnology，the improvement of tomato is performed by genetic engineefing method． Obtaining excellent resistance varieties has become the novel strategy and method of tomato molecular breeding．In the previous study，W砒W transcription factors play vital roles in plant disease resistance．However，research on the tomato is rarely reported．Screening of tomato WRKY transcription factors involving in disease resistance explore it functional mechanisms which provide the candidate genes for cultivate new varieties tomato of disease resistance and provide the practical evidence for further work of molecular breeding． In our laboratory，we have obtained WRKY gene，named印≯矿RK跖．Bioinformatics results predicated that the estimated molecular weight was 1 82．98 kDa．Its isoelectric point was 4．90．It was unstable hydrophobic protein composed by 739 amino acids．It did not harbor transmembrane region and signal peptide．A subcellular localization analysis predicated that the protein may exist in the nucleus．The SpWRKY6 protein contained two WRKY domains and C2H2一type zinc finger motifs．Therefore．it was classified as a group I WRKY transcription factors．The promoter analysis showed that sDWRKY6 has biotic and abiotic stress responsive elements as well as genevestigator online software predicted that it might be affected by biotic and abiotic s