强制转轮运动改善放射性认知功能障碍与相关的脑源性神经营养因子信号通路的分析.pdfVIP

强制转轮运动改善放射性认知功能障碍及相关的脑源性神经营养因子信号通路的研究 英文摘要 Forced running exercise attenuates hippocampal neurogenesis impairment and the neurocognitive deficits induced by whole-brain irradiation via the BDNF-mediated pathway Abstract Objective Radiation therapy is an important treatment modality for primary and metastatic head and neck tumors. Although white matter necrosis is uncommon with modern radiation therapy techniques, functional deficits have become increasingly important, having profound effects on quality of life. There are no proven successful long-term treatments or effective preventative strategies for radiation-induced cognitive impairment. Previous studies have indicated that exercise can improve cognitive function, and in which BDNF may play a very important role. In the present study, we investigated whether the running exercise after irradiation had the protective effects of the radiation-induced cognitive impairment and the changes in protein expression of BDNF signaling pathway in the hippocampus were also detected. Methods 64 healthy Sprague–Dawley rats (male, two month old) were divided into 4 groups: the control group, the low-intensity exercise group, the moderate-intensity exercise group, the high-intensity exercise group (n = 16 in each group). Animals in the exercise group were run on the motor-driven running wheel twice a day (30 min in the morning and 30 min in the afternoon), 5 days a week for a consecutive 3-week period. Behavioral tests were performed after exercise in the order of open field and Morris water maze. These tests were used to evaluate the anxiety level, hippocampal dependent spatial learning and memor