液氨预浸预处理甜菜渣提高纤维素酶解率生物工程专业论文.docxVIP

内蒙古大学硕士学位论文剂降解了48．95％，纤维素含量提高了85．96％，破坏半纤维素和木质素之间的连 内蒙古大学硕士学位论文 剂降解了48．95％，纤维素含量提高了85．96％，破坏半纤维素和木质素之间的连 接键。 3、液氨预浸预处理使得甜菜渣孔隙度增大，表面致密结构受到破坏，酶解 的有效比表面积增大，增加了纤维素酶与底物的酶解可及性；无定形纤维素受 到破坏，结晶度升高，纤维素结晶区暴露。 4、液氨预浸预处理破坏甜菜渣细胞壁，使得细胞间隙扩大，细胞大小发生 改变；相比未处理甜菜渣，细胞壁所产生的蓝色自发荧光均减弱，表明显著去 除木质素和半纤维素；破坏纤维结构，纤维表面变得粗糙并出现大量缝隙。 关键词：甜菜渣；液氨预浸；酶解率；化学组成；物理结构；微观形态 III 万方数据 内蒙古大学硕士学位论文Enhanced 内蒙古大学硕士学位论文 Enhanced Enzymatic Hydrolysis of Sugar Beet Pulp by Aqueous Ammonia Pretreatment ABSTRACT Cellulosic ethanol，with the advantages of renewable resource，‘cleanliness and safety，is the mainstream direction of new energy development and has been obtained extensive attention from countries and enterprises around the world，Sugar beet pulp， byproducts derived from sugar beet industry，is deemed as the potential feedstocks for the cellulosic ethanol production．However the low enzymatic hydrolysis of cellulose severely restricted the development of cellulosic ethanol production．Hence various pretreatments were established and used in ethanol production． In this study，aqueous ammonia pretreatment of sugar beet pulp at low temperature were established．The cellulose conversions of sugar beet pulp were analyzed．The effects of the pretreatrnent on the chemical composition，physical structure，and cell wall morphology were discussed． The effects hydrochloric acid pretreatment，aqueous ammonia pretreatment, ammonium oxalate pretreatment and pectinase pretreatment on cellulose conversions of sugar beet pulp were compared．The optimal process of aqueous ammonia pretreatment of sugar beet pulp at low temperature was established．The effects of 万方数据 内蒙古大学硕士学位论文the 内蒙古大学硕士学位论文 the pretreatment on the chemical composition，physical structure were discussed． And the changes of cell wall morphology were characterized by fluorescence microscopy，scanning electron microscopy，confocal laser scanning microscope in different level．The main conclusions ale as follows： 1．Aqueous ammonia pretreatment at low temperature was established．Th