表达牛乳铁蛋白肽的重组乳酸乳球菌对雏鸡生长的影响及抗鸡白痢沙门氏菌感染的效果观察预防兽医学专业论文.docxVIP

CoNTENTSCONTENTS CoNTENTS CONTENTS Abstract in Chinese ．I Abstract in English ． ．． ．．III 1 Introduction 1 1．1 The instruction for lactoferrin peptide ．1 1．1．1 Lactoferrcin molecular strucnlre 1 1．1．2 The biological function of lactofe：rrcin 1 1．1．3 The instruction for recombinant Lactococcus lactis expressing the bovine lactoferrin peptide 3 1．2 The instruction for Salmonella puUorum 3 1．3 Purpose and significance ofthe study ··4 2 Materials and Methods ．5 2．1 Materials 5 2．1．1 Strain and cell ．5 2．1．2 Experimental animal 5 2．1．3 Antibody preparation and kit 5 2．1．4 The major molecular biology reagent and medium 5 2．1．5 The prepare ofmain solution 一5 2．1．6 The main laboratory apparatus and equipment 6 2．2 Emprical method 7 2．2．1 Preparation of bovine lactoferrin monoclonal antibody ．．7 2．2．2 The identification ofRecombinant Lactococcus lactis pAMJ399-LFBA／MGl363 10 2．2．3 The promoting ofbovine lactoferrin peptide the growth of chicks 1 1 2．2．4 The protective e毹cts of recombinant Lactococcus lactis to chicks infected with Salmonella ． ． ．．12 3 Results ．15 3．1 The result of preparing bovine lactoferrin monoclonal antibody 1 5 3．1．1 The determination ofbest package antigen and antibody dilution 15 3．1．2 Establishment ofhybridoma cells 一15 3．1．3 Determination of MAb titer ．．1 5 3．1．4 Identification ofthe subtypes ofMAbs 16 3．1．5 Stability analysis ofmonoclonal antibody “16 3．1．6 Monoclonal antibody superimposed test ．16 3．1．7 The specifically reactive of bovine lactoferrin peptide with MAbs ·1 6 3．2 Results of identification of Recombinant Lactococcus lactis ．1 7 3．2．1 The identification results ofrecombinant plasmid pAMJ399-LFBA ．．17 3．2．2 Western—blot of expression product 17 III 万方数据 东北农业大学兽医硕士学位论文3．3 东北农业大学兽医硕士学位论文 3．3 The promoting ofbovine lactoferrin peptide the growth ofchicks 18 3．3．1 The effect ofbovine lactoferrin peptides growth performance ofchicks 18 3．3．2 The effect ofbovine lactoferrin peptide organ index ofchic