苹果腐烂病菌诱导产孢方法.PDFVIP

中国农学通报 2012,28(10): 151-154 Chinese Agricultural Science Bulletin 苹果腐烂病菌诱导产孢方法 赵 红，王彩霞，陈晓忍，王海艳，李保华 （青岛农业大学农学与植物保护学院/ 山东省植物病虫害综合防控重点实验室，山东青岛266109 ） 摘 要：为了研究一种能诱导苹果腐烂病菌快速大量产孢的方法，测试了6 种培养基对苹果腐烂病菌的 诱导产孢效果。结果发现，苹果腐烂病菌在加蜂蜜水和蛋白胨的带壳大麦上能大量产孢。接种苹果腐 烂病菌菌丝的带壳大麦，在黑暗中培养15 天后可产生大量黑色子实体。已形成子实体的大麦粒在黑光 灯下诱导25 天后开始产生分生孢子。每颗大麦粒平均产生11 个子实体，其中15%的子实体能溢出分生 6 孢子角。70 g 大麦粒上所产生的分生孢子可配制浓度为10 个孢子/mL 的孢子悬浮液1500~2000 mL 。 试验还发现，不同的腐烂病菌菌株在同一条件下产孢量存在很大差异。 关键词：苹果腐烂病；诱导产孢；大麦粒；分生孢子 中图分类号：S432.1 文献标志码：A 论文编号：2012-0025 Methods of Promoting Sporulation of Valsa ceratosperma Zhao Hong, Wang Caixia, Chen Xiaoren, Wang Haiyan, Li Baohua (College of Crop Protection and Agronomy, Qingdao Agricultural University/ Key Lab of Integrated Crop Pest Management of Shandong Province, Qingdao Shandong 266109) Abstract: In order to find methods to promote Valsa ceratosperma to produce enough conidia in short time, the effects of 6 kinds of culture medium were tested on promoting V. ceratosperma to produce conidia. The results showed that V. ceratosperma produced a large number of conidia in barley, which was with shell and soaked in solution of honey and peptone. After inoculated to the medium of barley and cultivated for about 15 days in the darkness, the fungi produced a large number of pycnidia. The pycnidia extruded conidia threads after cultivated under UV light for about 25 days. About 11 pycnidia produced on each barley grain on average and 15% of the pycnidia released conidia. The conidia produced by on 70 g barley would make 1500-2000 mL 6 conid