甘草酸协同麻黄碱的平喘作用机制研究-天津中草药杂志社.PDF

第 33 卷 第 3 期 2010 年 6 月 - 183 - 甘草酸协同麻黄碱的平喘作用机制研究 1 2 2 2 * 李若洁 ，石 倩 ，程彬峰 ，侯媛媛 1 南开大学 生命科学学院，天津 300071 2 南开大学 药学院，天津 300071 摘 要 目的：探讨甘草酸协同麻黄碱的平喘作用机制。方法：利用基于 CHO 细胞的 β 肾上腺素受体（β-AR ）激动药活 2 2 性评价体系，豚鼠离体气管收缩和组胺引喘的活体动物模型观察甘草酸和麻黄碱联用的协同平喘效果。结果：甘草 酸自身不能引起离体气管平滑肌舒张，平喘效果也不显著，但与麻黄碱联用可以通过提高 β2-AR 的激动效果，明 显增强平喘效果。结论：甘草酸通过调节 β2-AR 信号通路增强麻黄碱的作用效果，两者联用有良好的协同作用。 关键词 麻黄碱；甘草酸；β 肾上腺素受体激动药；糖皮质激素 2 中图分类号：R974.3 R969.2 文献标志码：A 文章编号：1674–6376 （2010 ）03–0183–04 Investigation of the anti-asthmatic mechanism of combination of glycyrrhizic acid and ephedrine 1 1 1 2 * LI Ruo-jie , SHI Qian , CHENG Bin-feng , HOU Yuan-yuan 1 College of Life Sciences, Nankai University, Tianjin 300071, China 2 College of Pharmacy, Nankai University, Tianjin 300071, China Abstract Objective: To investigate the anti-asthmatic mechanism of combination of glycyrrhizic acid and ephedrine. Methods: With cell evaluating systems of β adrenergic receptor (β-AR) agonists established by our laboratory, model of spasmolytic 2 2 activities in vitro and antitussive activities in vivo, the effect of combination use of glycyrrhizic acid and ephedrine was observed. Results: In our study, glycyrrhizic acid alone showed little anti-asthmatic effect and could not relax the airway smooth muscles. However, it could enhance the anti-asthmatic activity of ephedrine when these two compounds were used together. Conclusion: Glycyrrhizic acid could increase bronchodilation activity of ephedrine through β2-AR signal pathway