中国被毛孢菌丝体多糖提取结构表征及抗肿瘤活性研究生物化学与分子生物学专业论文.docxVIP

ABSTRACTCro，咖PJ6博Jj，?e”sf_is ABSTRACT Cro，咖PJ6博Jj，?e”sf_is kind of vaJuabIe medicinal funl三i in China and has mallv kinds of fbnctjons． P01ysaccharide of(、D，·dycP，，s Jf门P，，sis js of the main pharmacological actiVe ingredients．Polysaccharide of c白，z纱cElf，s J砌P，7s括has many kinds of biological actiVity whic|1 the antiLulllor， imInunological reguIatioll。 antidjabetjc a11d on．Its bjojogjcai activjty is JoseJy related to¨1e structure of polysaccharide，and the sIructure of polysaccharide is Vel了conlpIex，so the research and analysis of the stnlcture of p01ysacclla rIde lays foundation for the study of its biologicaI activity．This topic mainly researched(0，．咖Ef”J加口门s括which was preseⅣed in laborato哆．The nlain content and the resuIts of the research foIIowings： The identification of stIjain took advantage of the soIid cultivatioll．The activated strains cultured PDA under t11e dif琵rent temperature(16℃，18℃，20℃．22℃) for 30 days．Basis size of cololly diameteI．it was determined that t11e straill growtll op“mal tenlperatLlre was l 8℃．T11en the strain cultured solid cultivation at l 8℃ and observed morphological characteristics and size of coIony， nlycelIum and conidiulll．It was deterlllined that t|1e strain was the asexuaI tvpe of Cr口rdVc已口s si狂ens{s， The content of the polysaccharides ftonl nlvceliuJll was 4．7 l％士O．09％by usin2 the method of phenol—suIf．uric acid．The content of adenosine and cordycepin f卜om mycelium respcctiVely was 3．47士O．3 l 1119，g and I．32士0．19 mg／g by using t11e ITletllod of HPLC．The collIellt of the cordycepic acid from myce“um was 8．50％土0．05％by using method of uItraviolet spec【rophotonleter．T11e content of me protein“om InyceJjurn was 3 J．97％士O．08％by using tjle method of KjeJdahl．”1e contenI of the fat f’ronl myceIiuIn was 8．09％士O．89％by LIsing the 111ethod of soxhlet extraction．The results showed mat t11e active components of t11e artincial cu“ured mycelitjm were sjmiIar to naturaI cD，0we，坫J加P，埘旭and their contents were consistent witll t11e contentsofna“lr