低于抑制浓度的抗生素对铜绿假单胞菌基因表达的调节微生物学专业论文.docxVIP

西北大学硕十毕业论文检测，明确了这些化合物对基因表达的影响。该方法为从中药及其他天然成分中 西北大学硕十毕业论文 检测，明确了这些化合物对基因表达的影响。该方法为从中药及其他天然成分中 研究开发新型抗致病药物，提供了一个可行的技术平台。 在用低于抑制浓度的抗生素对启动子库检测的过程中，发现了一个受调节的 潜在转录调节子基因PA5283。基因敲除研究结果初步表明：PA5283基因对一些 毒性因子的表达在转录水平具有负调节作用，同时对QS系统相关基因(1asI, lasR、砌玑砌膻、pqsH和pqsR)具有不同程度的负调节。 关键词： 铜绿假单胞菌；毒性因子；低于抑制浓度的抗生素；基因调节；基因 敲除 西北大学硕上毕业论文Modulation 西北大学硕上毕业论文 Modulation of genes by subinhibitory concentrations of antibiotics in Pseudomonas aeruginosa Abstract Antibiotics have been co--only used鹤therapeutics to treat bacterial infections in humans and animals，and thus been perceived as bacterial killing or inhibiting agents in nature．However,recent studies have shown subinhibitory antibiotics play important roles in regulating bacterial genes including virulence factor genes．In this study,the expression of 1 3 secreted virulence related gene clusters of Pseudomonas aeruginosa，an important opportunistic pathogen，was examined in the presence of subinhibitory concentrations of four antibiotics using luxCDABE-based promoter- reporter fusion constructs．The results indicate that five ofthe 13 gene clusters，rhlAB， phzA2,lasB，exoY and exoS,were activated by all four sub-MlC antibiotics vancomycin，tetracycline，ampicilin and azythromycin；phzAl was also activated by them except ampicillin，in contrast to the previous observations that antibiotics at subinhibitory concentrations generally reduce bacterial virulence．Analysis of rhlAB showed I／4-MIC vancomycin resulted in 10一fold increase of its transcription． Rhaminolipid production that is carried out by rhaminosyltransferase encoded by rhlAB was also increased significantly,and so was pyocyanin production船well鹤P aeruginosa swarming and swimming motility．Similar results were observed with subinhibitory tetracycline，azithromycin and ampicillin．These results indicate that the antibiotics at low concentrations could up—regulate virulence factors and therefore influence bacterial pathogenesis，supporting the notion that antibiotics at low concentrations can ac