根皮素上调nrf2途径对大鼠脑缺血再灌注氧化应激损伤的神经保护及机制研究老年医学专业论文.docxVIP

脑缺血再灌注损伤的机制 脑缺血再灌注损伤的机制 ．．43 脑缺血再灌注损伤与氧化应激 一45 氧化应激指示酶 一47 核因子相关因子2 49 脑缺血再灌注损伤与氧化应激及Nrf2的相关性 ．50 根皮素结构特点、功能及与Nrf2相关性 ．5 l 局限 ．．56 展望 一57 结论 58 参考文献 ．．58 附图表 ．．70 综述：Nrf2调控下游基因HO．1抗氧化应激的研究 ．．79 致谢 ．．93 攻读学位论文期间发表学术论文目录 ．．94 附录已发表及待发表的英文文章 ．．95 万方数据 C0lNTENTSAbsl：I’act．．．． C0lNTENTS Absl：I’act．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．． ．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．1 Abstract in Chinese ． 2 Abstract in English ． ． ． ． ．5 Symbolic description ． ．．1 1 Introduction 13 Materials and methods ．1 6 Materials ．16 1．Animals ．．1 6 2．Drugs ．．1 7 3．Instruments and equipments 1 7 4．Reagents 1 7 Methods ．．1 8 1．Animal preparation 1 8 2．Animal groups 1 8 3．Preparation of intraluminal thread 1 9 4．Preparation ofmodels 19 5．Drug delivery method ．20 6．Evaluation of neurological deficit 20 7．Assessments of infarct volume 20 8．Measurements of brain edema ．． ．．2 1 9．Detection ofoxidative stress relating indicator ．．2 1 1 0．Reverse transcription polymerase chain reaction(RT-PCR) 30 1 1．Westem blot analysis ．34 Regults ．38 Discussions 41 Effects ofcerebral ischemia on brain tissue ． 41 Epidemiology ofcerebral vascular disease ．．42 Cerebral ischemia reperfusion injury ．．42 万方数据 Mechanism Mechanism of cerebral ischemia reperfusion injury．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．43 Cerebral ischemia reperfusion injury and oxidative stress． ．．．．．．．． ．．．．．．．．．．．．．．．．．．45 Oxidative stress indicator ． ． ． ．．47 Nuclear factor erythroid 2-related factor 2(Nrf2) ．．．49 Relationship between cerebral ischemia repe