阿托伐他汀对大鼠缺血心肌trail表达及细胞凋亡的影响内科心血管专业论文.docxVIP

温州医学院硕士学位论文阿托伐他汀对大鼠缺血心肌TRAI 温州医学院硕士学位论文 阿托伐他汀对大鼠缺血心肌TRAI L表达及细胞凋亡的影响 中文摘要 目的：观察阿托伐他汀对大鼠缺血心肌TRAIL表达的影响，以及与心肌细胞凋亡和凋亡相 关基因Bax，Bcl2的关系，探讨TRAIL因子在心血管疾病中所起的作用。 方法：采用左冠状动脉结扎的方法建立大鼠心肌梗死模型。健康雄性SD大鼠随机分为三 组：1)假手术组：2)心梗组：3)阿托伐他汀组(10mg／kg／d)。全自动生化仪分析血脂水平： 病理组织学测定心肌梗死面积：免疫组化及实时定量RT—PCR测定心肌TRAIL、Bax、Bcl一2 的表达：TUNEL法测定心肌细胞凋亡。 结果：心梗组凋亡细胞数显著增加，而阿托伐他汀干预显著减少梗死心肌细胞凋亡(P O．01)；心梗组Bcl一2蛋白表达升高，而阿托伐他汀干预梗死心肌Bel—2蛋白表达明显升 高(PO．05)；Bax蛋白表达在各组间均无明显差异：心梗组Bcl一2与Bax的比值下降， 阿托伐他汀干预明显升高(尺O．05)；心梗组TRAIL蛋白表达下降，而阿托伐他汀干预梗 死心肌TRAIL蛋白表达显著升高(尺O．01)。阿托伐他汀组Bcl-2基因水平较手术组及假 手术组升高(尸O．05)；心梗组Bax基因显著升高，而阿托伐他汀干预能明显降低梗死心 肌Bax的水平(／KO．05)；Bcl-2与Bax的比值在心梗组下降，而阿托伐他汀干预能升高 Bcl一2／Bax的比值(J|DO．05)：心梗组TRAIL基因表达下降，而阿托伐他汀干预梗死心肌 TRAIL基因表达显著升高(P0．01)。 结论：阿托伐他汀促使大鼠缺血心肌TRAIL基因表达上调，TRAIL基因可能参与了阿托伐他 汀抗心肌细胞凋亡过程。 [关键词] 阿托伐他汀；心肌缺血；TRAIL：大鼠 Study Study of Atorvastatin on TRAIL expression in the myocardiocytes and apoptosis in the rat with acute myocardial infarction ABSTRACT OBJECTIVE：To investigate the effect of atorvastatin on TRAIL expression in the mvocardiocytes and apoptosis in the rats with acute myocardial infarction(AMI)and the possible role of TRAIL in cardiovascular disease． METHODS：The rats with AMl were established by ligation of the left anterior descending coronary artery．Male Sprague—Dawley rats were randomly divided into three groups：1)the sham group；2)the untreated MI group；3)the group treated with Atorvastatin 1 0mg／kg／d·The level of lipid was analysised by automatic biochemical analyzer； infarction size was detemined by histomorphometric analysis；the expression of TRAIL，Bax and Bcl-2 in the myoeardium was measured by real time RT-PCR and immunohistochemistry；cardlomyocytes apoptosis was determined by TUNEL stain． RESULTS：The number of apoptotic cells significantly increased in the MI group and Atorvastatin markedly inhibited apoptosis in the myocardial after MI(P0．01)；the expression of Bcl．2 protein significantly increased in the MI group and Atorvastatin significantly increased Bcl．2 protein in the myocardial after MI