恩度联合紫杉醇不同时序给药对移植瘤小鼠血清vegf和hif1α水平影响的研究肿瘤学专业论文.docxVIP

也明显小于另外2个联合组(P值均O．05)。 也明显小于另外2个联合组(P值均O．05)。 结论：移植瘤小鼠血清中VEGF、HIF一1 Oc水平的变化与移植瘤组织微 血管改变密切相关。单一恩度抗血管效应短暂，血清VEGF、HIF—lot水平 的变化可反映抗血管效应的变化。在恩度用药期间，通过动态监测血清 VEGF、HIF一1 0【的变化，可更早的发现肿瘤微血管结构的改变，在血清 VEGF下降的初期联合给药可达到最佳的抗肿瘤效应。 关键词：内皮抑素类；紫杉醇；血管内皮生长因子；缺氧诱导因子 万方数据 Effect Effect of Recombinant human endostatin combined with Paclitaxel under different delivery order on serum HIF—l a and VEGF expression in mice xenografl tu mor m odel Abstract：Objective：Anti·-angiogenesis is the most important thing in anti·· tumor．Recombinant human endostatin(rh—endostatin)is China first anti—angi ogenesis targeted drugs，It is separated and purificated from e．coli engineering bacterium fermentation，and added nine amino acid residue s in the original End ostatin．make its traits more stable and higher activity．The anti angiogenesis eft ect of rh-endostatin could inhibit the proliferation and metastasis of tumor cells though reducing the migration and inducing apoptosis of the endothelial cells．S tudies have reported that when rh—endostatin combined with chemotherapy dru g，drug delivery sequence is different，the curative effect is also different．Seru m vascular endothelial growth factor(vascular endothelial groth factor，VEGF)， hypoxia inducing factor(hypoxia inducible factor 1 alpha and alpha HIF一1)hi gh level expression in a wide variety of tumor is closely related to tumor growt h and metastasis，but on the relationship with the antiangiogenesis effect is less study．This study was to observe the effect of recombinant human endostatin(r h-endostatin)combined with paclitaxel(PTX)under different delivery order on serum vascular endothelial groth factor(VEGF)and hypoxia inducible fac tor—l a(HIF—l a)expression in mice xenografl model，and to explore the applic 4 万方数据 ation ation value in anti—angiogenesis therapy． Methods：Subcutaneous xenograft tumor models of lung carcinoma Lewis cells were established in C57／BL mice，and the mice were randomly divided int 0 six groups：(1)Control group(intraperitonea